Long-term survival of Cryptococcus neoformans and Cryptococcus gattii in stored environmental samples from Colombia.
Background Both Cryptococcus neoformans and Cryptococcus gattii have been isolated from a variety of environmental sources in Colombia. Aim To determine the viability of C. neoformans / C. gattii isolates in stored soil samples, filtrates and bird droppings from which these yeasts were previously re...
| Publicado en: | Revista Iberoamericana de Micologia Vol. 32; no. 3; pp. 197 - 200 |
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| Autores principales: | , |
| Formato: | Artículo |
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Elsevier B.V.
Jul-Sep2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=109218934&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 109218934 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 11301406 PVP jtl: Revista Iberoamericana de Micologia issn: 11301406 maglogo: N pubinfo: dt: Jul-Sep2015 vid: 32 iid: 3 pid: 467 pub: Elsevier B.V. artinfo: ui: 109218934 10.1016/j.riam.2014.06.007 ppf: 197 ppct: 3 formats: fmt: @attributes: type: T tig: atl: Long-term survival of Cryptococcus neoformans and Cryptococcus gattii in stored environmental samples from Colombia. aug: au: Escandón, Patricia Castañeda, Elizabeth affil: Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, Colombia su: Cryptococcus Cryptococcus neoformans Environmental sampling Fungi conservation Fungal virulence Epidemiology Colombia sug: subj: Colombia Cryptococcus Cryptococcus neoformans Environmental sampling Fungi conservation Fungal virulence Epidemiology keyword: Cryptococcus gattii Environmental samples Muestras ambientales Supervivencia Survival Cryptococcus gattii Cryptococcus neoformans Environmental samples Muestras ambientales Supervivencia Survival ab: Background Both Cryptococcus neoformans and Cryptococcus gattii have been isolated from a variety of environmental sources in Colombia. Aim To determine the viability of C. neoformans / C. gattii isolates in stored soil samples, filtrates and bird droppings from which these yeasts were previously recovered. Methods A total of 964 samples collected between 2003 and 2009, and kept at room temperature were processed. From them, 653 samples were from trees decaying wood, 274 from soil filtrates and 37 from bird droppings. When C. neoformans or C. gattii were recovered, the molecular type of each isolate was established by PCR fingerprinting using the single primer (GTG) 5 . Results Among the processed samples, 161 isolates were recovered. From those, 81 (50.3%) corresponded to C. gattii recovered from decaying wood of Eucalyptus spp., Corymbia ficifolia , Terminalia catappa and Ficus spp. trees, and 80 (49.7%) corresponded to C. neoformans recovered from Ficus spp. and eucalyptus trees, as well as from bird droppings. The most prevalent molecular type among the C. gattii and C. neoformans isolates was VGII and VNI, respectively. Conclusions The re-isolation of C. neoformans / C. gattii from 10-year stored samples suggests that these yeasts are able to keep viable in naturally colonized samples. Resumen Antecedentes Cryptococcus neoformans y Cryptococcus gattii han sido aislados de diversas fuentes ambientales en Colombia. Objetivos Determinar la viabilidad de C. neoformans / C. gattii en muestras de suelo, filtrados y excrementos de aves en las que se habían aislado las levaduras previamente. Métodos Se procesaron 964 muestras recogidas entre los años 2003 y 2009, y almacenadas a temperatura ambiente. De estas, 653 muestras provenían de detritos, 274 de filtrados de suelo y 37 de excrementos de aves. Una vez recuperados C. neoformans y C. gattii , se determinó el patrón molecular mediante la técnica de PCR huella digital con el iniciador (GTG) 5 . Resultados Entre las muestras procesadas, se recuperaron 161 aislamientos. De estos, 81 (50,3%) fueron C. gattii recuperados de detritos de Eucalyptus spp., Corymbia ficifolia , Terminalia catappa y Ficus spp., y 80 (49,7%) eran C. neoformans recuperados de Ficus spp. y eucaliptos, así como de excrementos de aves. El patrón molecular más prevalente entre C. gattii y C. neoformans fue VGII y VNI, respectivamente. Conclusiones El reaislamiento de C. neoformans / C. gattii de muestras almacenadas durante 10 años evidencia que estas levaduras son capaces de mantenerse viables en muestras colonizadas naturalmente. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y custom: Copyright of Revista Iberoamericana de Micologia is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: Revista Iberoamericana de Micologia holder: Elsevier B.V. dt: @attributes: year: 2015 holdings: @attributes: islocal: N |
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