DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature.
Artificial gene synthesis requires consideration of nucleotide sequence development as well as long DNA molecule assembly protocols. The nucleotide sequence of the molecule must meet many conditions including particular preferences of the host organism for certain codons, avoidance of specific regul...
| Publicado en: | BioMed Research International Vol. 2015; pp. 1 - 9 |
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| Autores principales: | , , |
| Formato: | algorithm equations & formulas pictorial protocol research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
1/6/2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=109273035&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 109273035 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 1/6/2015 vid: 2015 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 109273035 109273035 109273035 10.1155/2015/413262 109273035 ppf: 1 ppct: 8 formats: fmt: @attributes: type: P tig: atl: DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature. aug: au: Nowak, Robert M. Wojtowicz-Krawiec, Anna Plucienniczak, Andrzej affil: Institute of Electronic Systems, Warsaw University of Technology, Nowowiejska 15/19, 00-665 Warsaw, Poland sug: subj: Software Utilization Genetic Engineering Methods DNA Nucleotides Algorithms Research Protocols Computer Simulation Systems Design Yeasts Peptide Hydrolases Polymerase Chain Reaction Data Analysis Software Funding Source ab: Artificial gene synthesis requires consideration of nucleotide sequence development as well as long DNA molecule assembly protocols. The nucleotide sequence of the molecule must meet many conditions including particular preferences of the host organism for certain codons, avoidance of specific regulatory subsequences, and a lack of secondary structures that inhibit expression. The chemical synthesis of DNA molecule has limitations in terms of strand length; thus, the creation of artificial genes requires the assembly of long DNA molecules from shorter fragments. In the approach presented, the algorithm and the computer program address both tasks: developing the optimal nucleotide sequence to encode a given peptide for a given host organism and determining the long DNA assembly protocol. These tasks are closely connected; a change in codon usage may lead to changes in the optimal assembly protocol, and the lack of a simple assembly protocol may be addressed by changing the nucleotide sequence. The computer program presented in this study was tested with real data from an experiment in a wet biological laboratory to synthesize a peptide. The benefit of the presented algorithm and its application is the shorter time, compared to polymerase cycling assembly, needed to produce a ready synthetic gene. pubtype: Academic Journal doctype: algorithm equations & formulas pictorial protocol research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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