Characterization of Red Blood Cells with Multiwavelength Transmission Spectroscopy.
Multiwavelength transmission (MWT) spectroscopy was applied to the investigation of the morphological parameters and composition of red blood cells (RBCs). The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonspherici...
| Publicado en: | BioMed Research International Vol. 2015; pp. 1 - 12 |
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| Autores principales: | , , |
| Formato: | equations & formulas research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
1/12/2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=109273065&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 109273065 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 1/12/2015 vid: 2015 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 109273065 109273065 109273065 10.1155/2015/382641 109273065 ppf: 1 ppct: 11 formats: fmt: @attributes: type: P tig: atl: Characterization of Red Blood Cells with Multiwavelength Transmission Spectroscopy. aug: au: Serebrennikova, Yulia M. Huffman, Debra E. Garcia-Rubio, Luis H. affil: Claro Scientific, LLC., 10100 Dr. Martin Luther King Jr. Street N., St. Petersburg, FL 33716, USA sug: subj: Erythrocytes Anatomy and Histology Spectroscopy, Near-Infrared Blood Chemical Analysis Anemia Diagnosis Erythrocytes Physiopathology Parasites P-Value Human T-Tests Malaria Linear Regression Data Analysis Software Correlation Coefficient Spectral Analysis Chemotherapy, Cancer ab: Multiwavelength transmission (MWT) spectroscopy was applied to the investigation of the morphological parameters and composition of red blood cells (RBCs). The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonsphericity and orientation of RBCs. The MWT spectra of the healthy and anemic samples were investigated for the RBC indices in open and blinded studies. When MWT performance was evaluated against a standard reference system, very good agreement between two methods, with R2>0.85 for all indices studied, was demonstrated. The RBC morphological parameters were used to characterize three types of anemia and to draw an association between RBC morphology and anemia severity. The MWT spectra of RBCs infected with malaria parasite Plasmodium falciparum at different life cycle stages were analyzed for RBC morphological parameters. The changes in the RBC volume, surface area, aspect ratio, and hemoglobin composition were used to trace the morphological and compositional alterations in the infected RBCs occurring with parasites’ development and to provide insights into parasite-host interactions. The MWT method was shown to be reliable for determination of the RBC morphological parameters and to be valuable for identification of the RBC pathologic changes and disease states. pubtype: Academic Journal doctype: equations & formulas research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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