Development, Characterization, and In Vitro Biological Performance of Fluconazole-Loaded Microemulsions for the Topical Treatment of Cutaneous Leishmaniasis.

Cutaneous leishmaniasis (CL) is a resistant form of leishmaniasis that is caused by a parasite belonging to the genus Leishmania. FLU-loaded microemulsions (MEs) were developed by phase diagram for topical administration of fluconazole (FLU) as prominent alternative to combat CL. Three MEs called F1...

Descripción completa

Detalles Bibliográficos
Publicado en:BioMed Research International Vol. 2015; pp. 1 - 13
Autores principales: Oliveira, Marcela Brito, Calixto, Giovana, Graminha, Márcia, Cerecetto, Hugo, González, Mercedes, Chorilli, Marlus
Formato: equations & formulas research tables/charts Journal Article
Publicado: Wiley-Blackwell 1/12/2015
Acceso en línea:Ver este registro en EBSCOhost
fields @attributes:
  recordID: 1
pdfLink:
plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=109273066&site=ehost-live
header:
  @attributes:
    shortDbName: ccm
    uiTerm: 109273066
    longDbName: CINAHL Complete
    uiTag: AN
  controlInfo:
    bkinfo:
    dissinfo:
    jinfo:
      jid:
        23146133
        FT2T
      jtl: BioMed Research International
      issn: 23146133
      maglogo: N
    pubinfo:
      dt: 1/12/2015
      vid: 2015
      pid: 480
      pub: Wiley-Blackwell
      place: Malden, Massachusetts
    artinfo:
      ui:
        109273066
        109273066
        109273066
        10.1155/2015/396894
        109273066
      ppf: 1
      ppct: 12
      formats:
        fmt:
          @attributes:
            type: P
      tig:
        atl: Development, Characterization, and In Vitro Biological Performance of Fluconazole-Loaded Microemulsions for the Topical Treatment of Cutaneous Leishmaniasis.
      aug:
        au:
          Oliveira, Marcela Brito
          Calixto, Giovana
          Graminha, Márcia
          Cerecetto, Hugo
          González, Mercedes
          Chorilli, Marlus
        affil: Department of Drugs and Medicines, School of Pharmaceutical Sciences, UNESP, Rodovia Araraquara-Jaú, km. 1, Campus, 14801-902 Araraquara, SP, Brazil
      sug:
        subj:
          Emulsions
          Fluconazole
          Leishmaniasis Drug Therapy
          Treatment Outcomes
          In Vitro Studies
          Fluconazole Administration and Dosage
          Fluconazole Pharmacodynamics
          Analysis of Variance
          Post Hoc Analysis
          P-Value
          Data Analysis Software
          Rheology
          Biological Assay
          Cholesterol
          Castor Oil
          Drug Delivery Systems
          Funding Source
      ab: Cutaneous leishmaniasis (CL) is a resistant form of leishmaniasis that is caused by a parasite belonging to the genus Leishmania. FLU-loaded microemulsions (MEs) were developed by phase diagram for topical administration of fluconazole (FLU) as prominent alternative to combat CL. Three MEs called F1, F2, and F3 (F1—60% 50 M phosphate buffer at pH 7.4 (PB) as aqueous phase, 10% cholesterol (CHO) as oil phase, and 30% soy phosphatidylcholine/oil polyoxyl-60 hydrogenated castor oil/sodium oleate (3/8/6) (S) as surfactant; F2—50% PB, 10% CHO, and 40% S; F3—40% PB, 10% CHO, and 50 % S) were characterized by droplet size analysis, zeta potential analysis, X-ray diffraction, continuous flow, texture profile analysis, and in vitro bioadhesion. MEs presented pseudoplastic flow and thixotropy was dependent on surfactant concentration. Droplet size was not affected by FLU. FLU-loaded MEs improved the FLU safety profile that was evaluated using red cell haemolysis and in vitro cytotoxicity assays with J-774 mouse macrophages. FLU-unloaded MEs did not exhibit leishmanicidal activity that was performed using MTT colourimetric assays; however, FLU-loaded MEs exhibited activity. Therefore, these MEs have potential to modulate FLU action, being a promising platform for drug delivery systems to treat CL.
      pubtype: Academic Journal
      doctype:
        equations & formulas
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
    refInfo:
    holdings:
      @attributes:
        islocal: N