Current and Emerging Cell Culture Manufacturing Technologies for Influenza Vaccines.

Annually, influenza virus infects millions of people worldwide. Vaccination programs against seasonal influenza infections require the production of hundreds of million doses within a very short period of time. The influenza vaccine is currently produced using a technology developed in the 1940s tha...

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Publicado en:BioMed Research International Vol. 2015; pp. 1 - 12
Autores principales: Milián, Ernest, Kamen, Amine A.
Formato: pictorial review tables/charts Journal Article
Publicado: Wiley-Blackwell 3/1/2015
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 3/1/2015
      vid: 2015
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1155/2015/504831
        109273511
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        atl: Current and Emerging Cell Culture Manufacturing Technologies for Influenza Vaccines.
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        au:
          Milián, Ernest
          Kamen, Amine A.
        affil: Department of Bioengineeringt, McGill University, Macdonald Engineering Building, 817 Sherbrooke Street W, Montreal, QC, Canada, H3A 0C3
      sug:
        subj:
          Drug Compounding Methods
          Cell Culture Techniques
          Technology, Pharmaceutical
          Drug Design Methods
          Influenza Vaccine Classification
          Influenza, Seasonal Prevention and Control
          Orthomyxoviridae Analysis
          Specimen Handling
          Genetic Engineering Methods
          Biotechnology Methods
          United States Food and Drug Administration
      ab: Annually, influenza virus infects millions of people worldwide. Vaccination programs against seasonal influenza infections require the production of hundreds of million doses within a very short period of time. The influenza vaccine is currently produced using a technology developed in the 1940s that relies on replicating the virus in embryonated hens’ eggs. The monovalent viral preparation is inactivated and purified before being formulated in trivalent or tetravalent influenza vaccines. The production process has depended on a continuous supply of eggs. In the case of pandemic outbreaks, this mode of production might be problematic because of a possible drastic reduction in the egg supply and the low flexibility of the manufacturing process resulting in a lack of supply of the required vaccine doses in a timely fashion. Novel production systems using mammalian or insect cell cultures have emerged to overcome the limitations of the egg-based production system. These industrially well-established production systems have been primarily selected for a faster and more flexible response to pandemic threats. Here, we review the most important cell culture manufacturing processes that have been developed in recent years for mass production of influenza vaccines.
      pubtype: Academic Journal
      doctype:
        pictorial
        review
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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