Improving the Thermostability and Optimal Temperature of a Lipase from the Hyperthermophilic Archaeon Pyrococcus furiosus by Covalent Immobilization.

A recombinant thermostable lipase (Pf2001Δ60) from the hyperthermophilic Archaeon Pyrococcus furiosus (PFUL) was immobilized by hydrophobic interaction on octyl-agarose (octyl PFUL) and by covalent bond on aldehyde activated-agarose in the presence of DTT at pH = 7.0 (one-point covalent attachment)...

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Publicado en:	BioMed Research International Vol. 2015; pp. 1 - 9
Autores principales:	Branco, Roberta V., Gutarra, Melissa L. E., Guisan, Jose M., Freire, Denise M. G., Almeida, Rodrigo V., Palomo, Jose M.
Formato:	equations & formulas pictorial research tables/charts Journal Article
Publicado:	Wiley-Blackwell 3/8/2015
Acceso en línea:	Ver este registro en EBSCOhost