The Mechanism of Melanocytes-Specific Cytotoxicity Induced by Phenol Compounds Having a Prooxidant Effect, relating to the Appearance of Leukoderma.
Specific phenol compounds including rhododendrol (RD), a skin-brightening ingredient in cosmetics, are reported to induce leukoderma, inducing a social problem, and the elucidation of mechanism of leukoderma is strongly demanded. This study investigated the relationship among the cytotoxicities of s...
| Publicado en: | BioMed Research International Vol. 2015; pp. 1 - 13 |
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| Autores principales: | , , , , |
| Formato: | equations & formulas pictorial research tables/charts tracings Journal Article |
| Publicado: |
Wiley-Blackwell
3/12/2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=109273629&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 109273629 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 3/12/2015 vid: 2015 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 109273629 109273629 109273629 10.1155/2015/479798 109273629 ppf: 1 ppct: 12 formats: fmt: @attributes: type: P tig: atl: The Mechanism of Melanocytes-Specific Cytotoxicity Induced by Phenol Compounds Having a Prooxidant Effect, relating to the Appearance of Leukoderma. aug: au: Nagata, Takeshi Ito, Shinobu Itoga, Kazuyoshi Kanazawa, Hideko Masaki, Hitoshi affil: Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 2-10 Kawadacho, Shinjuku-ku, Tokyo 162-0054, Japan sug: subj: Cytotoxins Phenols Reactive Oxygen Species Adverse Effects Skin Diseases Human Molecular Structure Chromatography, High Pressure Liquid Cells, Cultured In Vitro Studies Descriptive Statistics Data Analysis Software T-Tests Pearson's Correlation Coefficient Microscopy Magnetic Resonance Spectroscopy ab: Specific phenol compounds including rhododendrol (RD), a skin-brightening ingredient in cosmetics, are reported to induce leukoderma, inducing a social problem, and the elucidation of mechanism of leukoderma is strongly demanded. This study investigated the relationship among the cytotoxicities of six phenol compounds on B16F10 melanoma cells and HaCaT keratinocytes and generated reactive oxygen species (ROS). As a result, the cytotoxicity of RD on B16F10 cells was higher than that on HaCaT cells, and RD significantly increased intracellular ROS and hydrogen peroxide (H2O2) levels in B16F10 cells. Furthermore, although raspberry ketone (RK), RD derivative, also increased intracellular ROS in B16F10 cells, increase in ROS was suppressed by disodium dihydrogen ethylenediaminetetraacetate dehydrate (EDTA). The amounts of increased ROS with RK in HaCaT cells without melanocyte were further increased by tyrosinase. Therefore, tyrosinase, a metalloprotein having copper, was speculated to be one of causative agents allowing phenol compounds to work as a prooxidant. Hydroxyl radical was generated by adding a mixture of tyrosinase and H2O2 to RD, and the amount of the radical was further increased by UVB, indicating that RD cytotoxicity was caused by intracellularly increased ROS, which possibly related to phenol induced prooxidants. pubtype: Academic Journal doctype: equations & formulas pictorial research tables/charts tracings Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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