Redesigning Protein Cavities as a Strategy for Increasing Affinity in Protein-Protein Interaction: Interferon- γ Receptor 1 as a Model.
Combining computational and experimental tools, we present a new strategy for designing high affinity variants of a binding protein. The affinity is increased by mutating residues not at the interface, but at positions lining internal cavities of one of the interacting molecules. Filling the cavitie...
| Publicado en: | BioMed Research International Vol. 2015; pp. 1 - 13 |
|---|---|
| Autores principales: | , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
4/28/2015
|
| Acceso en línea: | Ver este registro en EBSCOhost |