Preparation and Characterization of Solid Dispersions of Artemether by Freeze-Dried Method.

Solid dispersions of artemether and polyethylene glycol 6000 (PEG6000) were prepared in ratio 12 : 88 (group-1). Self-emulsified solid dispersions of artemether were prepared by using polyethylene glycol 6000, Cremophor-A25, olive oil, Transcutol, and hydroxypropyl methylcellulose (HPMC) in ratio 12...

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Publicado en:BioMed Research International Vol. 2015; pp. 1 - 12
Autores principales: Ansari, Muhammad Tayyab, Hussain, Altaf, Nadeem, Sumaira, Majeed, Humaira, Saeed-Ul-Hassan, Syed, Tariq, Imran, Mahmood, Qaisar, Khan, Abida Kalsoom, Murtaza, Ghulam
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 5/17/2015
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 5/17/2015
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      pub: Wiley-Blackwell
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        10.1155/2015/109563
        109274217
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        atl: Preparation and Characterization of Solid Dispersions of Artemether by Freeze-Dried Method.
      aug:
        au:
          Ansari, Muhammad Tayyab
          Hussain, Altaf
          Nadeem, Sumaira
          Majeed, Humaira
          Saeed-Ul-Hassan, Syed
          Tariq, Imran
          Mahmood, Qaisar
          Khan, Abida Kalsoom
          Murtaza, Ghulam
        affil: Faculty of Pharmacy, Bahauddin Zakariya University, Multan 6000, Pakistan
      sug:
        subj:
          Freeze Drying
          Chemistry, Pharmaceutical Methods
          Malaria Drug Therapy
          Human
          One-Way Analysis of Variance
          Microscopy, Electron, Scanning
          Research Methodology
      ab: Solid dispersions of artemether and polyethylene glycol 6000 (PEG6000) were prepared in ratio 12 : 88 (group-1). Self-emulsified solid dispersions of artemether were prepared by using polyethylene glycol 6000, Cremophor-A25, olive oil, Transcutol, and hydroxypropyl methylcellulose (HPMC) in ratio 12 : 75 : 5 : 4 : 2 : 2, respectively (group-2). In third group, only Cremophor-A25 was replaced with Poloxamer 188 compared to group-2. The solid dispersions and self-emulsified solid dispersions were prepared by physical and freeze dried methods, respectively. All samples were characterized by X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, differential scanning calorimeter, scanning electron microscopy, and solubility, dissolution, and stability studies. X-ray diffraction pattern revealed artemether complete crystalline, whereas physical mixture and freeze-dried mixture of all three groups showed reduced peak intensities. In attenuated total reflectance Fourier transform infrared spectroscopy spectra, C–H stretching vibrations of artemether were masked in all prepared samples, while C–H stretching vibrations were representative of polyethylene glycol 6000, Cremophor-A25, and Poloxamer 188. Differential scanning calorimetry showed decreased melting endotherm and increased enthalpy change (ΔH) in both physical mixture and freeze-dried mixtures of all groups. Scanning electron microscopy of freeze-dried mixtures of all samples showed glassy appearance, size reduction, and embedment, while their physical mixture showed size reduction and embedment of artemether by excipients. In group-1, solubility was improved up to 15 times, whereas group-2 showed up to 121 times increase but, in group-3, when Poloxamer 188 was used instead of Cremophor-A25, solubility of freeze-dried mixtures was increased up to 135 times. In fasted state simulated gastric fluid at pH 1.6, the dissolution of physical mixture was increased up to 12 times and freeze-dried mixtures up to 15 times. The stability of artemether was substantially enhanced in freeze-dried mixtures by using polyethylene glycol 6000, Cremophor-A25, and Poloxamer 188 of self-emulsified solid dispersions of artemether in Hank’s balanced salt solution at pH 7.4.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
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        Journal Article
      ougenre: Article
    language: English
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