Bucky Tubes Induce Oxidative Stress Mediated Cell Death in Human Lung Cells.
Unique physicochemical properties of carbon nanomaterials (CNMs) have opened a new era for therapeutics and diagnosis (known as theranostics) of various diseases. This exponential increase in application makes them important for toxicology studies. The present study was aimed at exploring the toxic...
| Publicado en: | BioMed Research International Vol. 2015; pp. 1 - 14 |
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| Autores principales: | , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
5/18/2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=109274285&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 109274285 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 5/18/2015 vid: 2015 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 109274285 109274285 109274285 10.1155/2015/560768 109274285 ppf: 1 ppct: 13 formats: fmt: @attributes: type: P tig: atl: Bucky Tubes Induce Oxidative Stress Mediated Cell Death in Human Lung Cells. aug: au: Singhal, Jaya Singh, Surinder P. Karuppiah, Stalin Pandey, Alok K. affil: Nanomaterial Toxicology Laboratory, CSIR, Indian Institute of Toxicology Research, P. O. Box 80, Mahatma Gandhi Marg, Lucknow 226001, India sug: subj: Oxidative Stress Cell Death Lung Neoplasms Physiopathology Nanostructures Carbon Lung Neoplasms Etiology Toxicity Tests Human Flow Cytometry Reactive Oxygen Species Biological Markers Lipid Peroxidation Mitochondria Apoptosis Cell Cycle One-Way Analysis of Variance Descriptive Statistics Post Hoc Analysis Funding Source ab: Unique physicochemical properties of carbon nanomaterials (CNMs) have opened a new era for therapeutics and diagnosis (known as theranostics) of various diseases. This exponential increase in application makes them important for toxicology studies. The present study was aimed at exploring the toxic potential of one of the CNMs, that is, bucky tubes (BTs), in human lung adenocarcinoma (A549) cell line. BTs were characterised by electron microscopy (TEM), dynamic light scattering (DLS), Fourier transform spectroscopy (FTIR), and X-ray diffraction (XRD). Flow cytometric study showed a concentration and time dependent increase in intracellular internalization as well as reduction in cell viability upon exposure to BTs. However, a significant increase in intracellular reactive oxygen species (ROS) production was observed as evident by increased fluorescence intensity of 2′,7′-dichlorofluorescein (DCF). BTs induced oxidative stress in cells as evident by depletion in glutathione with concomitant increase in lipid peroxidation with increasing concentrations. A significant increase in micronucleus formation and apoptotic cell population and loss of mitochondrial membrane potential (MMP) as compared to control were observed. Moreover, in the present study, BTs were found to be mild toxic and it is encouraging to conclude that BTs having outer diameter in the range of 7–12 nm and length 0.5–10 μm can be used for theranostics. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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