Steroid Anti-Inflammatory Effects Did Not Improve Organ Quality in Brain-Dead Rats.
Effect of glucocorticoid administration on improving the outcomes of kidney and liver allografts has not been clearly elucidated. This study investigated the effect of prednisolone administration after onset of brain death (BD) on kidney and liver in a controlled rat model of BD. BD was induced in r...
| Publicado en: | BioMed Research International Vol. 2015; pp. 1 - 11 |
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| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
5/19/2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=109274320&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 109274320 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 5/19/2015 vid: 2015 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 109274320 109274320 109274320 10.1155/2015/207534 109274320 ppf: 1 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Steroid Anti-Inflammatory Effects Did Not Improve Organ Quality in Brain-Dead Rats. aug: au: Rebolledo, Rolando A. Liu, Bo Akhtar, Mohammed Z. Ottens, Petra J. Zhang, Jian-ning Ploeg, Rutger J. Leuvenink, Henri G. D. affil: Department of Surgery, University Medical Center Groningen, 9713 GZ Groningen, Netherlands sug: subj: Prednisolone Pharmacodynamics Kidney Drug Effects Liver Drug Effects Brain Death Animal Studies Rats Prednisolone Administration and Dosage Inflammation Gene Expression Apoptosis Interleukins Blood Creatinine Blood Aspartate Aminotransferase Blood Lactate Dehydrogenase Blood Prednisolone Adverse Effects Enzyme-Linked Immunosorbent Assay Polymerase Chain Reaction Immunohistochemistry Kruskal-Wallis Test Tumor Necrosis Factor Blood Transplant Donors Prednisolone Therapeutic Use Hemodynamics Drug Effects Organ Transplantation ab: Effect of glucocorticoid administration on improving the outcomes of kidney and liver allografts has not been clearly elucidated. This study investigated the effect of prednisolone administration after onset of brain death (BD) on kidney and liver in a controlled rat model of BD. BD was induced in rats by inflating an epidurally placed balloon catheter. Animals were treated with saline or prednisolone (5, 12.5, or 22.5 mg/kg) one hour after the onset of BD. After 4 hours of BD, experiments were terminated and serum and tissues were collected. Tissue gene and protein expression were measured for markers of inflammation, apoptosis, and cellular stress response markers. Prednisolone caused a reduction of plasma levels of IL-6, while the tissue expression of IL-6, IL-1β, and MCP-1 in both kidney and liver were also reduced. Creatinine plasma levels, complement (C3) expression, HSP-70, HO-1, Bcl2/BAX ratio, and PMN influx did not significantly change in kidney nor liver. Plasma AST and LDH levels were increased in the prednisolone treated group. Our results demonstrate prednisolone can has an anti-inflammatory effect mediated through reducing serum circulating cytokines. However, this anti-inflammatory effect does not translate into improved kidney function and indeed was associated with increased liver injury markers. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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