Lipase-Secreting Bacillus Species in an Oil-Contaminated Habitat: Promising Strains to Alleviate Oil Pollution.

Lipases are of great interest for different industrial applications due to their diversity and versatility. Among different lipases, microbial lipases are preferable due to their broad substrate specificity, and higher stability with lower production costs compared to the lipases from plants and ani...

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Publicado en:BioMed Research International Vol. 2015; pp. 1 - 10
Autores principales: Lee, Li Pin, Karbul, Hudzaifah Mohamed, Citartan, Marimuthu, Gopinath, Subash C. B., Lakshmipriya, Thangavel, Tang, Thean-Hock
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 6/9/2015
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 6/9/2015
      vid: 2015
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1155/2015/820575
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        atl: Lipase-Secreting Bacillus Species in an Oil-Contaminated Habitat: Promising Strains to Alleviate Oil Pollution.
      aug:
        au:
          Lee, Li Pin
          Karbul, Hudzaifah Mohamed
          Citartan, Marimuthu
          Gopinath, Subash C. B.
          Lakshmipriya, Thangavel
          Tang, Thean-Hock
        affil: Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, 13200 Kepala Batas, Penang, Malaysia
      sug:
        subj:
          Petroleum
          Water Pollution
          Lipase Therapeutic Use
          Bacillus
          Oils Adverse Effects
          Lipase
          Funding Source
          Cost Benefit Analysis
          Environmental Health
          Peptide Hydrolases Therapeutic Use
          Industrial Waste Adverse Effects
      ab: Lipases are of great interest for different industrial applications due to their diversity and versatility. Among different lipases, microbial lipases are preferable due to their broad substrate specificity, and higher stability with lower production costs compared to the lipases from plants and animals. In the past, a vast number of bacterial species have been reported as potential lipases producers. In this study, the lipases-producing bacterial species were isolated from an oil spillage area in the conventional night market. Isolated species were identified as Bacillus species by biochemical tests which indicate their predominant establishment, and further screened on the agar solid surfaces using lipid and gelatin as the substrates. Out of the ten strains tested, four potential strains were subjected to comparison analysis of the lipolytic versus proteolytic activities. Strain 10 exhibited the highest lipolytic and proteolytic activity. In all the strains, the proteolytic activity is higher than the lipolytic activity except for strain 8, suggesting the possibility for substrate-based extracellular gene induction. The simultaneous secretion of both the lipase and protease is a mean of survival. The isolated bacterial species which harbour both lipase and protease enzymes could render potential industrial-based applications and solve environmental issues.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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