Epitope Fingerprinting for Recognition of the Polyclonal Serum Autoantibodies of Alzheimer's Disease.
Autoantibodies (aAb) associated with Alzheimer's disease (AD) have not been sufficiently characterized and their exact involvement is undefined. The use of information technology and computerized analysis with phage display technology was used, in the present research, to map the epitope of putative...
| Published in: | BioMed Research International Vol. 2015; pp. 1 - 9 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Published: |
Wiley-Blackwell
8/31/2015
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=109471163&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 109471163 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 8/31/2015 vid: 2015 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 109471163 109471163 NLM26417591 10.1155/2015/267989 NLM26417591 PMC4568325 109471163 ppf: 1 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Epitope Fingerprinting for Recognition of the Polyclonal Serum Autoantibodies of Alzheimer's Disease. aug: au: de Oliveira-Júnior, Luiz Carlos Araújo Santos, Fabiana de Almeida Goulart, Luiz Ricardo Ueira-Vieira, Carlos affil: Laboratório de Genética, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Rua Acre s/n, Bloco 2E sala 230, Campus Umuarama, 38400-902 Uberlândia, MG, Brazil sug: ab: Autoantibodies (aAb) associated with Alzheimer's disease (AD) have not been sufficiently characterized and their exact involvement is undefined. The use of information technology and computerized analysis with phage display technology was used, in the present research, to map the epitope of putative self-antigens in AD patients. A 12-mer random peptide library, displayed on M13 phages, was screened using IgG from AD patients with two repetitions. Seventy-one peptides were isolated; however, only 10 were positive using the Elisa assay technique (Elisa Index > 1). The results showed that the epitope regions of the immunoreactive peptides, identified by phage display analysis, were on the exposed surfaces of the proteins. The putative antigens MAST1, Enah, MAO-A, X11/MINT1, HGF, SNX14, ARHGAP 11A, APC, and CENTG3, which have been associated with AD or have functions in neural tissue, may indicate possible therapeutic targets. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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