Effects of prostaglandin E1 on callus formation in rabbits.

Background: Recent research has focused on identifying chemical modulators of osteogenesis. We present initial findings on the osteoinductive properties of prostaglandin Е1 (Vasaprostan), using a rabbit model.Methods: Data were collected on callus formation in 14 male rabbits. These were divided int...

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Publicado en:BMC Musculoskeletal Disorders Vol. 16; no. 1; pp. 1 - 8
Autores principales: Lipinsky, Pawel V., Sirotin, Ivan V., Skoroglyadov, Alexandr V., Ivkov, Alexey V., Oettinger, Alexandr P., Krynetskiy, Evgeny E., But-Gusaim, Alexandr B., Roth, Andreas J.
Formato: diagnostic images pictorial research Journal Article
Publicado: BioMed Central 9/11/2015
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 9/11/2015
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      pub: BioMed Central
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        atl: Effects of prostaglandin E1 on callus formation in rabbits.
      aug:
        au:
          Lipinsky, Pawel V.
          Sirotin, Ivan V.
          Skoroglyadov, Alexandr V.
          Ivkov, Alexey V.
          Oettinger, Alexandr P.
          Krynetskiy, Evgeny E.
          But-Gusaim, Alexandr B.
          Roth, Andreas J.
        affil: Traumatology, Orthopaedics Department, 64 Clinical City Hospital, Russian State Medical University, Vavilova 61, 117292 Moscow, Russia
      sug:
        subj:
          Tibia Radiography
          Callus Radiography
          Vasodilator Agents Administration and Dosage
          Tibia Injuries
          Fractures Drug Therapy
          Fractures Radiography
          Animal Studies
          Callus Drug Effects
          Male
          Rabbits
          Injections, Subcutaneous
          Treatment Outcomes
          Male
      ab: Background: Recent research has focused on identifying chemical modulators of osteogenesis. We present initial findings on the osteoinductive properties of prostaglandin Е1 (Vasaprostan), using a rabbit model.Methods: Data were collected on callus formation in 14 male rabbits. These were divided into two groups (control and treatment) with 7 animals in each group. In all animals, the right tibia was fractured using a standardized protocol and stabilized by an intramedullary nail. Treatment group received a 5 μg/kg subcutaneous injection of PGE1/day during 10 postoperative days. Visual and radiological evaluation of callus formation was prospectively collected. After 30 days, all animals were killed and the tibia specimens were examined histologically.Results: In all the treatment group animals, fractures were consolidated radiologically by day 30. No treatment group animals and two control group animals were excluded form the experiment. In the control group, 4 animals demonstrated slower callus formation than the main group. Two control group animals were excluded from the experiment on the 20th day due to wound infection; one developed a nonunion. The mean coefficient of bone callus thickening in the main group was 2.08 (±0, 16) and 1.77 (±0.05) (p < 0.05) in the control group. Calculation of mean quantity of neogenic vessels in 10 random visual fields of the bone callus revealed 78 (±9.82) in the main group and 40 (±4.68) in the control group (p < 0.05).Conclusions: Our study demonstrates an increased rate and amount of bone callus formation in the group treated with prostaglandin E1 compared to the control group. Prospective radiological analysis was corroborated by histologic evaluation.
      pubtype: Academic Journal
      doctype:
        diagnostic images
        pictorial
        research
        Journal Article
      ougenre: Article
    language: English
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