Optical and analytical investigations on dengue virus rapid diagnostic test for IgM antibody detection.
Evaluation of binding between analytes and its relevant ligands on surface plasmon resonance (SPR) biosensor is of considerable importance for accurate determination and screening of an interference in immunosensors. Dengue virus serotype 2 was used as a case study in this investigation. This resear...
| Publicado en: | Medical & Biological Engineering & Computing Vol. 53; no. 8; pp. 679 - 688 |
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| Autores principales: | , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Aug2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=109582364&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 109582364 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01400118 PO0 jtl: Medical & Biological Engineering & Computing issn: 01400118 maglogo: N pubinfo: dt: Aug2015 vid: 53 iid: 8 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 109582364 NLM25791696 2013057300 10.1007/s11517-015-1262-2 NLM25791696 109582364 ppf: 679 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Optical and analytical investigations on dengue virus rapid diagnostic test for IgM antibody detection. aug: au: Jahanshahi, Peyman Sekaran, Shamala Devi Adikan, Faisal Rafiq Mahamd sug: ab: Evaluation of binding between analytes and its relevant ligands on surface plasmon resonance (SPR) biosensor is of considerable importance for accurate determination and screening of an interference in immunosensors. Dengue virus serotype 2 was used as a case study in this investigation. This research work compares and interprets the results obtained from analytical analysis with the experimental ones. Both the theoretical calculations and experimental results are verified with one sample from each category of dengue serotypes 2 (low, mid, and high positive), which have been examined in the database of established laboratorial diagnosis. In order to perform this investigation, the SPR angle variations are calculated, analyzed, and then validated via experimental SPR angle variations. Accordingly, the error ratios of 5.35, 6.54, and 3.72% were obtained for the low-, mid-, and high-positive-specific immune globulins of patient serums, respectively. In addition, the magnetic fields of the biosensor are numerically simulated to show the effect of different binding mediums. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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