Comparison between modified Dixon MRI techniques, MR spectroscopic relaxometry, and different histologic quantification methods in the assessment of hepatic steatosis.

Objectives: To compare systematically quantitative MRI, MR spectroscopy (MRS), and different histological methods for liver fat quantification in order to identify possible incongruities.Methods: Fifty-nine consecutive patients with liver disorders were examined on a 3 T MRI system. Quantitative MRI...

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Published in:European Radiology Vol. 25; no. 10; pp. 2869 - 2880
Main Authors: Kukuk, Guido M, Hittatiya, Kanishka, Sprinkart, Alois M, Eggers, Holger, Gieseke, Jürgen, Block, Wolfgang, Moeller, Philipp, Willinek, Winfried A, Spengler, Ulrich, Trebicka, Jonel, Fischer, Hans-Peter, Schild, Hans H, Träber, Frank
Format: research Journal Article
Published: Springer Nature Oct2015
Online Access:View this record in EBSCOhost
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      dt: Oct2015
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      pub: Springer Nature
      place: New York, New York
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        2013163932
        10.1007/s00330-015-3703-6
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        atl: Comparison between modified Dixon MRI techniques, MR spectroscopic relaxometry, and different histologic quantification methods in the assessment of hepatic steatosis.
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          Kukuk, Guido M
          Hittatiya, Kanishka
          Sprinkart, Alois M
          Eggers, Holger
          Gieseke, Jürgen
          Block, Wolfgang
          Moeller, Philipp
          Willinek, Winfried A
          Spengler, Ulrich
          Trebicka, Jonel
          Fischer, Hans-Peter
          Schild, Hans H
          Träber, Frank
      sug:
        subj:
          Fatty Liver Pathology
          Prospective Studies
          Epithelial Cells Pathology
          Middle Age
          Histological Techniques
          Aged
          Young Adult
          Adolescence
          Adult
          Female
          Male
          Magnetic Resonance Imaging Methods
          Human
          Magnetic Resonance Spectroscopy Methods
          Software
          Validation Studies
          Comparative Studies
          Evaluation Research
          Multicenter Studies
          Scales
          Middle Aged: 45-64 years
          Aged: 65+ years
          Adolescent: 13-18 years
          Adult: 19-44 years
          Female
          Male
      ab: Objectives: To compare systematically quantitative MRI, MR spectroscopy (MRS), and different histological methods for liver fat quantification in order to identify possible incongruities.Methods: Fifty-nine consecutive patients with liver disorders were examined on a 3 T MRI system. Quantitative MRI was performed using a dual- and a six-echo variant of the modified Dixon (mDixon) sequence, calculating proton density fat fraction (PDFF) maps, in addition to single-voxel MRS. Histological fat quantification included estimation of the percentage of hepatocytes containing fat vesicles as well as semi-automatic quantification (qHisto) using tissue quantification software.Results: In 33 of 59 patients, the hepatic fat fraction was >5% as determined by MRS (maximum 45%, mean 17%). Dual-echo mDixon yielded systematically lower PDFF values than six-echo mDixon (mean difference 1.0%; P < 0.001). Six-echo mDixon correlated excellently with MRS, qHisto, and the estimated percentage of hepatocytes containing fat vesicles (R = 0.984, 0.967, 0.941, respectively, all P < 0.001). Mean values obtained by the estimated percentage of hepatocytes containing fat were higher by a factor of 2.5 in comparison to qHisto. Six-echo mDixon and MRS showed the best agreement with values obtained by qHisto.Conclusions: Six-echo mDixon, MRS, and qHisto provide the most robust and congruent results and are therefore most appropriate for reliable quantification of liver fat.Key Points: • Six-echo mDixon correlates excellently with MRS, qHisto, and the estimated percentage of fat-containing hepatocytes. • Six-echo mDixon, MRS, and qHisto provide the most robust and congruent results. • Dual-echo mDixon yields systematically lower PDFF values than six-echo mDixon. • The percentage of fat-containing hepatocytes is 2.5-fold higher than fat fraction determined by qHisto. • Performance characteristics and systematic differences of the various methods should be considered.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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