Distribution of Myosin Attachment Times Predicted from Viscoelastic Mechanics of Striated Muscle.
We demonstrate that viscoelastic mechanics of striated muscle, measured as elastic and viscous moduli, emerge directly from the myosin crossbridge attachment time, tatt, also called time-on. The distribution of tatt was modeled using a gamma distribution with shape parameter, p, and scale parameter,...
| Publicado en: | Journal of Biomedicine & Biotechnology pp. 1 - 17 |
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| Autores principales: | , , |
| Formato: | Journal Article |
| Publicado: |
Wiley-Blackwell
2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=109652688&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 109652688 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11107243 137K jtl: Journal of Biomedicine & Biotechnology issn: 11107243 maglogo: N pubinfo: dt: 2011 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 109652688 2011452894 NLM22190855 PMC3228685 109652688 ppf: 1 ppct: 16 formats: fmt: @attributes: type: P tig: atl: Distribution of Myosin Attachment Times Predicted from Viscoelastic Mechanics of Striated Muscle. aug: au: Palmer, Bradley M. Yuan Wang Miller, Mark S. affil: Department of Molecular Physiology and Biophysics, University of Vermont, 122 HSRF, Beaumont Avenue, Burlington, VT 05405, USA sug: ab: We demonstrate that viscoelastic mechanics of striated muscle, measured as elastic and viscous moduli, emerge directly from the myosin crossbridge attachment time, tatt, also called time-on. The distribution of tatt was modeled using a gamma distribution with shape parameter, p, and scale parameter, β. At 5mM MgATP, β was similar between mouse α-MyHC (16.0 ± 3.7ms) and β-MyHC (17.9 ± 2.0ms), and p was higher (P < 0.05) for β-MyHC (5.6 ± 0.4 no units) compared to α-MyHC (3.2 ± 0.9). At 1mMMgATP, p approached a value of 10 in both isoforms, but β rose only in the β-MyHC (34.8 ± 5.8ms). The estimated mean tatt (i.e., pβ product) was longer in the β-MyHC compared to α-MyHC, and became prolonged in both isoforms as MgATP was reduced as expected. The application of our viscoelastic model to these isoforms and varying MgATP conditions suggest that tatt is better modeled as a gamma distribution due to its representing multiple temporal events occurring within tatt compared to a single exponential distribution which assumes only one temporal event within tatt. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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