PET imaging of cardiac wound healing using a novel [68Ga]-labeled NGR probe in rat myocardial infarction.
Purpose: Peptides containing the asparagine-glycine-arginine (NGR) motif bind to aminopeptidase N (CD13), which is expressed on inflammatory cells, endothelial cells, and fibroblasts. It is unclear whether radiolabeled NGR-containing tracers could be used for in vivo imaging of the early wound-heali...
| Publicado en: | Molecular Imaging & Biology Vol. 17; no. 1; pp. 76 - 87 |
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| Autores principales: | , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Feb2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=109693783&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 109693783 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Feb2015 vid: 17 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 109693783 NLM25011975 2012875251 10.1007/s11307-014-0751-2 NLM25011975 109693783 ppf: 76 ppct: 11 formats: fmt: @attributes: type: P tig: atl: PET imaging of cardiac wound healing using a novel [68Ga]-labeled NGR probe in rat myocardial infarction. aug: au: Tillmanns, Jochen Schneider, Magdalena Fraccarollo, Daniela Schmitto, Jan-Dieter Länger, Florian Richter, Dominik Bauersachs, Johann Samnick, Samuel sug: ab: Purpose: Peptides containing the asparagine-glycine-arginine (NGR) motif bind to aminopeptidase N (CD13), which is expressed on inflammatory cells, endothelial cells, and fibroblasts. It is unclear whether radiolabeled NGR-containing tracers could be used for in vivo imaging of the early wound-healing phase after myocardial infarction (MI) using positron emission tomography (PET).Procedures: Uptake of novel tracer [(68)Ga]NGR was assessed together with [(68)Ga]arginine-glycine-aspartic acid ([(68)Ga]RGD) and 2-deoxy-2-[(18) F]fluoro-D-glucose after myocardial ischemia/reperfusion (MI/R) injury using μ-PET and autoradiography, and relative expressions of CD13 and integrin β3 were assessed in fibroblasts, inflammatory cells, and endothelial cells by immunohistochemistry.Results: In the infarcted myocardium, uptake of [(68)Ga]NGR was maximal from days 3 to 7 after MI/R, and correlated with fibroblast and inflammatory cell infiltration as well as [(68)Ga]RGD uptake.Conclusions: [(68)Ga]NGR allows noninvasive and sequential determination of CD13 expression in fibroblasts and inflammatory cells by PET. This will facilitate monitoring of CD13 in the individual wound healing processes, allowing patient-specific therapies to improve outcome after MI. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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