PIXSIC, a pixelated β⁺-sensitive probe for radiopharmacological investigations in rat brain: binding studies with [¹⁸F]MPPF.

Purpose: The aim of this work was to demonstrate the pharmacokinetic potential of a wireless pixelated β(+)-sensitive probe (PIXSIC).Procedures: The binding of 2'-methoxyphenyl-(N-2'-pyridinyl)-p-[(18)F]fluoro-benzamidoethylpiperazine ([(18)F]MPPF), a 5-HT1A serotonin receptor radiopharmaceutical, w...

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Publicado en:Molecular Imaging & Biology Vol. 17; no. 2; pp. 163 - 168
Autores principales: Balasse, L, Maerk, J, Pain, F, Genoux, A, Fieux, S, Morel, C, Gisquet-Verrier, P, Zimmer, L, Lanièce, P
Formato: research Journal Article
Publicado: Springer Nature Apr2015
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Apr2015
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      pub: Springer Nature
      place: New York, New York
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        10.1007/s11307-014-0785-5
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        atl: PIXSIC, a pixelated β⁺-sensitive probe for radiopharmacological investigations in rat brain: binding studies with [¹⁸F]MPPF.
      aug:
        au:
          Balasse, L
          Maerk, J
          Pain, F
          Genoux, A
          Fieux, S
          Morel, C
          Gisquet-Verrier, P
          Zimmer, L
          Lanièce, P
      sug:
        subj:
          Pyridines
          Heterocyclic Compounds
          Brain
          Radiography
          Cerebellum
          Kinetics
          Animal Studies
          Injections, Intravenous
          Tomography, Emission-Computed
          Rats
          Wireless Communications
          Hippocampus
          Comparative Studies
          Multicenter Studies
          Evaluation Research
          Validation Studies
          Scales
      ab: Purpose: The aim of this work was to demonstrate the pharmacokinetic potential of a wireless pixelated β(+)-sensitive probe (PIXSIC).Procedures: The binding of 2'-methoxyphenyl-(N-2'-pyridinyl)-p-[(18)F]fluoro-benzamidoethylpiperazine ([(18)F]MPPF), a 5-HT1A serotonin receptor radiopharmaceutical, was measured in anesthetized rats and compared to microPET data. The effects of a 5-HT1A antagonist injection on in vivo [(18)F]MPPF binding were monitored by PIXSIC.Results: PIXSIC allowed differentiating the radioactive kinetics according to the location of its pixels in the hippocampus, cortex, corpus callosum, and cerebellum. The device accurately detected the changes in [(18)F]MPPF binding, after 5-HT1A antagonist blockade. The time-activity curves were reproducible and consistent with kinetics obtained simultaneously with a microPET camera.Conclusions: These results demonstrate the ability of the PIXSIC device to record reliably the binding of PET ligands, with a high spatiotemporal resolution in anesthetized rodents. These first in vivo results are a key stage on the path to its implementation in awake freely moving animals.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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