Bacterial and fungal keratitis in Upper Egypt: in vitro screening of enzymes, toxins and antifungal activity.
Purpose: This work was conducted to study the ability of bacterial and fungal isolates from keratitis cases in Upper Egypt to produce enzymes, toxins, and to test the isolated fungal species sensitivity to some therapeutic agents.Materials and Methods: One hundred and fifteen patients clinically dia...
| Publicado en: | Indian Journal of Ophthalmology Vol. 62; no. 2; pp. 196 - 204 |
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| Autores principales: | , , , |
| Formato: | research Journal Article |
| Publicado: |
Wolters Kluwer India Pvt Ltd
Feb2014
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=109751008&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 109751008 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03014738 1BRZ jtl: Indian Journal of Ophthalmology issn: 03014738 maglogo: N pubinfo: dt: Feb2014 vid: 62 iid: 2 pid: 16919 pub: Wolters Kluwer India Pvt Ltd artinfo: ui: 109751008 NLM24008795 2012505853 10.4103/0301-4738.116463 NLM24008795 PMC4005237 109751008 ppf: 196 ppct: 8 formats: tig: atl: Bacterial and fungal keratitis in Upper Egypt: in vitro screening of enzymes, toxins and antifungal activity. aug: au: Gharamah, Abdullah A Moharram, Ahmed M Ismail, Mady A Al-Hussaini, Ashraf K sug: subj: Antibiotics Therapeutic Use Antifungal Agents Therapeutic Use Bacterial Toxins Metabolism Eye Infections, Bacterial Epidemiology Eye Infections, Fungal Epidemiology Keratitis Epidemiology Mycotoxins Bacteria Drug Effects Bacteria Bacteria Metabolism Cornea Microbiology Egypt Eye Infections, Bacterial Drug Therapy Eye Infections, Bacterial Microbiology Eye Infections, Fungal Drug Therapy Eye Infections, Fungal Microbiology Fungi Drug Effects Fungi Fungi Metabolism Human Incidence Keratitis Drug Therapy Keratitis Microbiology ab: Purpose: This work was conducted to study the ability of bacterial and fungal isolates from keratitis cases in Upper Egypt to produce enzymes, toxins, and to test the isolated fungal species sensitivity to some therapeutic agents.Materials and Methods: One hundred and fifteen patients clinically diagnosed to have microbial keratitis were investigated. From these cases, 37 bacterial isolates and 25 fungal isolates were screened for their ability to produce extra-cellular enzymes in solid media. In addition, the ability of fungal isolates to produce mycotoxins and their sensitivity to 4 antifungal agents were tested.Results: Protease, lipase, hemolysins, urease, phosphatase, and catalase were detected respectively in 48.65%, 37.84%, 59.46%, 43.24%, 67.57%, and 100% out of 37 bacterial isolates tested. Out of 25 fungal isolates tested during the present study, 80% were positive for protease, 84% for lipase and urease, 28% for blood hemolysis, and 100% for phosphatase and catalase enzymes. Thirteen fungal isolates were able to produce detectable amounts of 7 mycotoxins in culture medium (aflatoxins (B1, B2, G1, and G2), sterigmatocystin, fumagillin, diacetoxyscirpenol, zearalenone, T-2 toxin, and trichodermin). Among the antifungal agents tested in this study, terbinafine showed the highest effect against most isolates in vitro.Conclusion: In conclusion, the ability of bacterial and fungal isolates to produce extracellular enzymes and toxins may be aid in the invasion and destruction of eye tissues, which, in turn, lead to vision loss. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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