DMP1-derived peptides promote remineralization of human dentin.
Remineralization of dentin during dental caries is of considerable clinical interest. Dentin matrix protein 1 (DMP1) is a non-collagenous calcium-binding protein that plays a critical role in biomineralization. In the present study, we tested if peptides derived from DMP1 can be used for dentin remi...
| Publicado en: | Journal of Dental Research Vol. 94; no. 4; pp. 608 - 615 |
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| Autores principales: | , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Sage Publications Inc.
Apr2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=109778697&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 109778697 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00220345 1H7 jtl: Journal of Dental Research issn: 00220345 maglogo: Y pubinfo: dt: Apr2015 vid: 94 iid: 4 pid: 344 pub: Sage Publications Inc. place: Thousand Oaks, California artinfo: ui: 109778697 NLM25694469 2012947730 10.1177/0022034515572441 NLM25694469 PMC4485220 109778697 ppf: 608 ppct: 7 formats: tig: atl: DMP1-derived peptides promote remineralization of human dentin. aug: au: Padovano, J D Ravindran, S Snee, P T Ramachandran, A Bedran-Russo, A K George, A affil: Brodie Tooth Development Genetics & Regenerative Medicine Research Laboratory, Department of Oral Biology, University of Illinois at Chicago, Chicago, IL, USA. sug: subj: Dentin Drug Effects Proteins Pharmacodynamics Phosphoproteins Pharmacodynamics Tooth Remineralization Industry Calcium Pharmacodynamics Calcium Binding Proteins Pharmacodynamics Collagen Metabolism Crystallography Dental Caries Metabolism Dental Caries Pathology Dentin Metabolism Dentin Minerals Metabolism Microscopy, Electron, Scanning Microscopy, Electron Peptides Pharmacodynamics Phosphates Pharmacodynamics Glycoproteins Pharmacodynamics Time Factors X-Rays ab: Remineralization of dentin during dental caries is of considerable clinical interest. Dentin matrix protein 1 (DMP1) is a non-collagenous calcium-binding protein that plays a critical role in biomineralization. In the present study, we tested if peptides derived from DMP1 can be used for dentin remineralization. Peptide pA (pA, MW = 1.726 kDa) and peptide pB (pB, MW = 2.185), containing common collagen-binding domains and unique calcium-binding domains, were synthesized by solid-phase chemistry. An extreme caries lesion scenario was created by collagenase digestion, and the biomineral-nucleating potential of these peptides was ascertained when coated on collagenase-treated dentin matrix and control, native human dentin matrix under physiological levels of calcium and phosphate. Scanning electron microscopy analysis suggests that peptide pB was an effective nucleator when compared with pA. However, a 1:4 ratio of pA to pB was determined to be ideal for dentin remineralization, based on hydroxyapatite (HA) morphology and calcium/phosphorus ratios. Interestingly, HA was nucleated on collagenase-challenged dentin with as little as 20 min of 1:4 peptide incubation. Electron diffraction confirmed the presence of large HA crystals that produced a diffraction pattern indicative of a rod-like crystal structure. These findings suggest that DMP1-derived peptides may be useful to modulate mineral deposition and subsequent formation of HA when exposed to physiological concentrations of calcium and phosphate. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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