Fingerprint analysis of processed Rhizoma Chuanxiong by high-performance liquid chromatography coupled with diode array detection.
Background: Rhizoma Chuanxiong (RC) is the dried rhizome of Ligusticum chuanxiong Hort., and various types of processed Rhizoma Chuanxiong (PRC) are widely used in China. However, quality assurance and quality control of these processed medicines remain challenging. This study aims to investigate th...
| Publicado en: | Chinese Medicine Vol. 10; no. 1; pp. 1 - 8 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | equations & formulas research tables/charts Journal Article |
| Publicado: |
BioMed Central
2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=109789360&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 109789360 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17498546 78JI jtl: Chinese Medicine issn: 17498546 maglogo: N pubinfo: dt: 2015 vid: 10 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 109789360 102805399 10.1186/s13020-015-0031-3 109789360 ppf: 1 ppct: 7 formats: tig: atl: Fingerprint analysis of processed Rhizoma Chuanxiong by high-performance liquid chromatography coupled with diode array detection. aug: au: Jia-Yan Fang Lin Zhu Tao Yi Jian-Ye Zhang Ling Yi Zhi-Tao Liang Li Xia Jia-Fu Feng Jun Xu Yi-Na Tang Zhong-Zhen Zhao Hu-Biao Chen affil: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong Special Administrative Region, Hong Kong, People's Republic of China sug: subj: Plants, Medicinal Therapeutic Use Chromatography, Liquid Medicine, Chinese Traditional Human Technology Electricity Biochips Drugs, Chinese Herbal DNA Fingerprinting Indicators and Reagents ab: Background: Rhizoma Chuanxiong (RC) is the dried rhizome of Ligusticum chuanxiong Hort., and various types of processed Rhizoma Chuanxiong (PRC) are widely used in China. However, quality assurance and quality control of these processed medicines remain challenging. This study aims to investigate the chemical compositions of various PRC preparations by a high-performance liquid chromatography (HPLC) coupled with diode array detection (DAD) method. Methods: A HPLC-DAD method with validation was developed for PRC samples. Seven batches of plant samples from two processing methods, stir-frying and steaming, were analyzed by the HPLC-DAD method. Common peaks in PRC chromatograms were chosen to calculate their relative retention time (RRT) and relative peak area (RPA), and similarity analyses of the chromatographic fingerprints were conducted by Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine software (Version 2004 A). Results: In the 24-h stability test, the relative standard deviation for the RRT and RPA was less than 0.07% and 2.57%, respectively. The precision was less than 0.08% for the RRT and 2.48% for the RPA. The repeatability for the RRT and RPA was less than 0.03% and 2.64%, respectively. The similarities between the seven PRC batches were range from 0.956 to 0.990. After stir-frying or steaming, the amount of ferulic acid in PRC was much higher than that in the raw material. Conclusions: The fingerprint analysis of PRC by different processing methods was feasible by HPLC-DAD. pubtype: Academic Journal doctype: equations & formulas research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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