Phyllanthus spp. Exerts Anti-Angiogenic and Anti-Metastatic Effects Through Inhibition on Matrix Metalloproteinase Enzymes.

Tumor angiogenesis and metastasis are the major causes for high morbidity and mortality rates in cancer patient. Modulation on tumor angiogenesis and metastasis provides opportunities to halt progression of cancer. From our previous findings,Phyllanthusplant possesses antiproliferative effects on me...

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Published in:Nutrition & Cancer Vol. 67; no. 5; pp. 783 - 796
Main Authors: Tang, Yin-Quan, Jaganath, Indu Bala, Manikam, Rishya, Sekaran, Shamala Devi
Format: research tables/charts Journal Article
Published: Taylor & Francis Ltd Jul2015
Online Access:View this record in EBSCOhost
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      dt: Jul2015
      vid: 67
      iid: 5
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      pub: Taylor & Francis Ltd
      place: Philadelphia, Pennsylvania
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        10.1080/01635581.2015.1040518
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        atl: Phyllanthus spp. Exerts Anti-Angiogenic and Anti-Metastatic Effects Through Inhibition on Matrix Metalloproteinase Enzymes.
      aug:
        au:
          Tang, Yin-Quan
          Jaganath, Indu Bala
          Manikam, Rishya
          Sekaran, Shamala Devi
        affil: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
      sug:
        subj:
          Neovascularization, Pathologic
          Plants, Medicinal Therapeutic Use
          Matrix Metalloproteinases
          Nutrition
          Vascular Endothelial Growth Factor A
          Neoplasm Metastasis
          Melanoma Prevention and Control
          Prostatic Neoplasms Prevention and Control
          Male
          Plant Extracts
          Melanoma Therapy
          Prostatic Neoplasms Therapy
          Cell Culture Techniques
          Endothelium
          Cell Migration Inhibition
          Data Analysis
          Data Analysis Software
          T-Tests
          Cell Movement
          Male
      ab: Tumor angiogenesis and metastasis are the major causes for high morbidity and mortality rates in cancer patient. Modulation on tumor angiogenesis and metastasis provides opportunities to halt progression of cancer. From our previous findings,Phyllanthusplant possesses antiproliferative effects on melanoma and prostate cancer cell lines and induction of apoptosis. The main aims of the present work were further investigated on the antimetastatic and antiangiogenic effects on cancer cells (MeWo and PC-3) and human umbilical vein endothelial cells (HUVECs) of 4Phyllanthusspecies (P.amarus, P.niruri, P.urinariaandP.watsonii).Phyllanthusextracts significantly inhibited cell adhesion, migration, invasion, and transendothelial migration activities of cancer (MeWo and PC-3) cells in a dose-dependent manner (P< 0.05) by cell-matrix adhesion, Transwell migration, invasion, and transendothelial migration assays.Phyllanthusextracts were exhibited low cytotoxicity on HUVECs up to a concentration of 500.0 μg/ml by MTS reduction assay.Phyllanthusextracts also exhibited antiangiogenic effects through inhibition of migration, invasion, and microcapillary like-tube structure formation in HUVECs. These observations were due to alteration in activities of matrix metalloproteinase (MMP) −2, −7, −9, and −26 in treated-endothelial and cancer cells by zymographies. These findings suggest thatPhyllanthusplant has the potential to inhibit tumour metastasis and angiogenesis through the suppression of MMP enzymes.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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