An immunohistochemistry study of sox9, runx2, and osterix expression in the mandibular cartilages of newborn mouse.
The purpose of this study is to investigate the spacial expression pattern and functional significance of three key transcription factors related to bone and cartilage formation, namely, Sox9, Runx2, and Osterix in cartilages during the late development of mouse mandible. Immunohistochemical examina...
| Publicado en: | BioMed Research International Vol. 2013; pp. 265380 - 265381 |
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| Autores principales: | , , , , |
| Formato: | research Journal Article |
| Publicado: |
Wiley-Blackwell
2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=109857916&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 109857916 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2013 vid: 2013 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 109857916 2012152563 10.1155/2013/265380 NLM23762831 PMC3671271 109857916 ppf: 265380 ppct: 1 formats: fmt: @attributes: type: P tig: atl: An immunohistochemistry study of sox9, runx2, and osterix expression in the mandibular cartilages of newborn mouse. aug: au: Zhang, Hong Zhao, Xiaopeng Zhang, Zhiguang Chen, Weiwei Zhang, Xinli affil: Department of Orthodontics, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou 510055, China ; Dental and Craniofacial Research Institute, School of Dentistry, University of California, Los Angeles, CA, 90095, USA. sug: subj: Cartilage Metabolism Mandible Metabolism Proteins Metabolism Transcription Factors Metabolism Animal Population Groups Animal Studies Cartilage Chondrocytes Chondrocytes Metabolism Immunohistochemistry Mandible Mice Models, Biological Osteoblasts Osteoblasts Metabolism ab: The purpose of this study is to investigate the spacial expression pattern and functional significance of three key transcription factors related to bone and cartilage formation, namely, Sox9, Runx2, and Osterix in cartilages during the late development of mouse mandible. Immunohistochemical examinations of Sox9, Runx2, and Osterix were conducted in the mandibular cartilages of the 15 neonatal C57BL/6N mice. In secondary cartilages, both Sox9 and Runx2 were weakly expressed in the polymorphic cell zone, strongly expressed in the flattened cell zone and throughout the entire hypertrophic cell zone. Similarly, both transcriptional factors were weakly expressed in the uncalcified Meckel's cartilage while strongly expressed in the rostral cartilage. Meanwhile, Osterix was at an extremely low level in cells of the flattened cell zone and the upper hypertrophic cell zone in secondary cartilages. Surprisingly, Osterix was intensely expressed in hypertrophic chondrocytes in the center of the uncalcified Meckel's cartilage while moderately expressed in part of hypertrophic chondrocytes in the rostral process. Consequently, it is suggested that Sox9 is a main and unique positive regulator in the hypertrophic differentiation process of mandibular secondary cartilages, in addition to Runx2. Furthermore, Osterix is likely responsible for phenotypic conversion of Meckel's chondrocytes during its degeneration. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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