Kidney-targeted transplantation of mesenchymal stem cells by ultrasound-targeted microbubble destruction promotes kidney repair in diabetic nephropathy rats.
We test the hypothesis that ultrasound-targeted microbubble destruction (UTMD) technique increases the renoprotective effect of kidney-targeted transplantation of bone-marrow-derived mesenchymal stem cells (BM-MSCs) in diabetic nephropathy (DN) rats. Diabetes was induced by streptozotocin injection...
| Published in: | BioMed Research International Vol. 2013; pp. 526367 - 526368 |
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| Main Authors: | , , , , , , , , , |
| Format: | research Journal Article |
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Wiley-Blackwell
2013
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=109857935&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 109857935 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2013 vid: 2013 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 109857935 2012152582 10.1155/2013/526367 NLM23762850 PMC3677660 109857935 ppf: 526367 ppct: 1 formats: fmt: @attributes: type: P tig: atl: Kidney-targeted transplantation of mesenchymal stem cells by ultrasound-targeted microbubble destruction promotes kidney repair in diabetic nephropathy rats. aug: au: Zhang, Yi Ye, Chuan Wang, Gong Gao, Yunhua Tan, Kaibin Zhuo, Zhongxiong Liu, Zheng Xia, Hongmei Yang, Dan Li, Peijing affil: Department of Ultrasound, Xinqiao Hospital of the Third Military Medical University, 183 Xinqiaozheng Road, Chongqing 400037, China ; Department of Ultrasound, The Forty-Fourth Military Hospital, 67 Huanghe Road, Guiyang 550009, China. sug: subj: Connective Tissue Cells Diabetic Nephropathies Pathology Diabetic Nephropathies Therapy Equipment and Supplies Kidney Pathology Stem Cells Transplantation Ultrasonics Animal Studies Antigens, Surface Antigens, Surface Metabolism Blood Glucose Metabolism Blotting, Western Bone Marrow Capillary Permeability Cells, Cultured Diabetes Mellitus Diabetes Mellitus Blood Diabetes Mellitus Pathology Diabetes Mellitus Therapy Diabetic Nephropathies Diabetic Nephropathies Blood Enzyme-Linked Immunosorbent Assay Genes Immunohistochemistry Insulin Blood Male Models, Biological Pancreas Pathology Rats RNA RNA Metabolism Staining and Labeling Male ab: We test the hypothesis that ultrasound-targeted microbubble destruction (UTMD) technique increases the renoprotective effect of kidney-targeted transplantation of bone-marrow-derived mesenchymal stem cells (BM-MSCs) in diabetic nephropathy (DN) rats. Diabetes was induced by streptozotocin injection (60 mg/Kg, intraperitoneally) in Sprague-Dawley rats. MSCs were administered alone or in combination with UTMD to DN rats at 4 weeks after diabetes onset. Random blood glucose concentrations were measured at 1, 2, 4, and 8 weeks, and plasma insulin levels, urinary albumin excretion rate (UAER) values, the structures of pancreas and kidney, the expressions of TGF-β1, synaptopodin, and IL-10 were assessed at 8 weeks after MSCs transplantation. MSCs transplantation decreased blood glucose concentrations and attenuated pancreatic islets/β cells damage. The permeability of renal interstitial capillaries and VCAM-1 expression increased after UTMD, which enhanced homing and retention of MSCs to kidneys. MSCs transplantation together with UTMD prevented renal damage and decreased UAER values by inhibiting TGF-β1 expression and upregulating synaptopodin and IL-10 expression. We conclude that MSCs transplantation reverts hyperglycemia; UTMD technique noninvasively increases the homing of MSCs to kidneys and promotes renal repair in DN rats. This noninvasive cell delivery method may be feasible and efficient as a novel approach for personal MSCs therapy to diabetic nephropathy. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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