The influence of micronutrients in cell culture: a reflection on viability and genomic stability.
Micronutrients, including minerals and vitamins, are indispensable to DNA metabolic pathways and thus are as important for life as macronutrients. Without the proper nutrients, genomic instability compromises homeostasis, leading to chronic diseases and certain types of cancer. Cell-culture media tr...
| Publicado en: | BioMed Research International Vol. 2013; pp. 597282 - 597283 |
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| Autores principales: | , , , , , , |
| Formato: | review Journal Article |
| Publicado: |
Wiley-Blackwell
2013
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=109858335&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 109858335 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2013 vid: 2013 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 109858335 2012159783 10.1155/2013/597282 NLM23781504 PMC3678455 109858335 ppf: 597282 ppct: 1 formats: fmt: @attributes: type: P tig: atl: The influence of micronutrients in cell culture: a reflection on viability and genomic stability. aug: au: Arigony, Ana Lúcia Vargas de Oliveira, Iuri Marques Machado, Miriana Bordin, Diana Lilian Bergter, Lothar Prá, Daniel Pêgas Henriques, Joao Antonio affil: Departamento de Biofísica/Centro de Biotecnologia, UFRGS, Campus do Vale, Porto Alegre, RS, Brazil. sug: subj: Cell Culture Techniques Methods Mutation Drug Effects Micronutrients Animals Cell Physiology Drug Effects Culture Media Pharmacodynamics ab: Micronutrients, including minerals and vitamins, are indispensable to DNA metabolic pathways and thus are as important for life as macronutrients. Without the proper nutrients, genomic instability compromises homeostasis, leading to chronic diseases and certain types of cancer. Cell-culture media try to mimic the in vivo environment, providing in vitro models used to infer cells' responses to different stimuli. This review summarizes and discusses studies of cell-culture supplementation with micronutrients that can increase cell viability and genomic stability, with a particular focus on previous in vitro experiments. In these studies, the cell-culture media include certain vitamins and minerals at concentrations not equal to the physiological levels. In many common culture media, the sole source of micronutrients is fetal bovine serum (FBS), which contributes to only 5-10% of the media composition. Minimal attention has been dedicated to FBS composition, micronutrients in cell cultures as a whole, or the influence of micronutrients on the viability and genetics of cultured cells. Further studies better evaluating micronutrients' roles at a molecular level and influence on the genomic stability of cells are still needed. pubtype: Academic Journal doctype: review Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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