Isolation and characterization of Omithobacteriun rhinotracheale from respiratory diseased turkeys.

Ornithobacterium rhinotracheale, a Gram-negative bacterium, was isolated from 8- week-old turkeys with respiratory disease. The flock suffered 30% mortality and the main lesions included pneumonia and liver congestion. Both isolates had a biocode: 0-2-2-0-0-0-4 according to the commercial API 20NE s...

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Detalles Bibliográficos
Publicado en:	Veterinaria México Vol. 34; no. 3; pp. 283 - 289
Autores principales:	Vargas, Edgardo Soriano, Garduño, Manuel Longinoa, Rosas, Pomposo Fernández
Formato:	Artículo
Publicado:	Facultad de Medicina Veterinaria y Zootecnia UNAM jul-sep2003
Materias:	Turkeys Gram-negative bacteria Gram-negative bacterial diseases Bacteria Respiratory diseases Diseases
Acceso en línea:	Ver este registro en EBSCOhost

Descripción
Sumario:	Ornithobacterium rhinotracheale, a Gram-negative bacterium, was isolated from 8- week-old turkeys with respiratory disease. The flock suffered 30% mortality and the main lesions included pneumonia and liver congestion. Both isolates had a biocode: 0-2-2-0-0-0-4 according to the commercial API 20NE system, and agglutinated chicken glutaraldehyde-fixed erythrocytes. Likewise, isolates were recognized by an antisera raised against a previously typed O. rhinotracheale isolate by plate agglutination and inhibition-hemagglutination tests. Isolates were included in an ERIC-PCR assay, showing the same amplicon patterns that were isolated from two broilers and one layer with O. rhinotracheale. Results obtained indicate that O. rhinotracheale could be an important agent in respiratory disease outbreaks in Mexican turkey farms. Se aisló Ornithobacterium rhinotracheale, bacteria gramnegativa, de pavos de ocho semanas de edad con enfermedad respiratoria. La parvada sufrió 30%. de mortalidad y las principales lesiones incluyeron neumonía y congestión hepática. Ambos aislamientos tuvieron el biocódigo: 0-2-2-0-0-0-4 del sistema comercial API 20NE y aglutinaron eritrocitos de pollo fijados con glutaraldehído. Asimismo, los aislamientos fueron reconocidos por un antisuero elaborado para un aislamiento previamente tipificado de O. rhinotracheale mediante pruebas de aglutinación en placa e inhibición de la hemoaglutinación. Los aislamientos se incluyeron en un ensayo de ERIC-PCR, que mostró el mismo patrón de amplicones que el de dos aislamientos de O. rhinotracheale obtenido de pollos de engorda y uno de gallinas de postura. Los resultados obtenidos indican que O, rhinotracheale puede ser un agente importante de brotes de enfermedad respiratoria en explotaciones de pavos en México.