Fuchs Endothelial Corneal Dystrophy: Strong Association with rs613872 Not Paralleled by Changes in Corneal Endothelial TCF4 mRNA Level.

Fuchs endothelial corneal dystrophy (FECD) is a common corneal endotheliopathy with a complex and heterogeneous genetic background. Different variants in the TCF4 gene have been strongly associated with the development of FECD. TCF4 encodes the E2-2 transcription factor but the link between the stro...

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Publicado en:BioMed Research International Vol. 2015; pp. 1 - 7
Autores principales: Ołdak, Monika, Ruszkowska, Ewelina, Udziela, Monika, Oziębło, Dominika, Bińczyk, Ewelina, Ścieżyńska, Aneta, Płoski, Rafał, Szaflik, Jacek P.
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 9/16/2015
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 9/16/2015
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        atl: Fuchs Endothelial Corneal Dystrophy: Strong Association with rs613872 Not Paralleled by Changes in Corneal Endothelial TCF4 mRNA Level.
      aug:
        au:
          Ołdak, Monika
          Ruszkowska, Ewelina
          Udziela, Monika
          Oziębło, Dominika
          Bińczyk, Ewelina
          Ścieżyńska, Aneta
          Płoski, Rafał
          Szaflik, Jacek P.
        affil: Department of Genetics, World Hearing Center, Institute of Physiology and Pathology of Hearing, Warsaw, Poland
      sug:
        subj:
          Corneal Dystrophies, Hereditary Physiopathology
          Human
          Funding Source
          Corneal Dystrophies, Hereditary Familial and Genetic
          Genes
          Polymorphism, Genetic
          Genotype
          Alleles
          RNA
          Polymerase Chain Reaction
          Chi Square Test
          Odds Ratio
          Confidence Intervals
          Unpaired T-Tests
          Data Analysis Software
          Pearson's Correlation Coefficient
          Male
          Female
          Case Control Studies
          Goodness of Fit Chi Square Test
          Male
          Female
      ab: Fuchs endothelial corneal dystrophy (FECD) is a common corneal endotheliopathy with a complex and heterogeneous genetic background. Different variants in the TCF4 gene have been strongly associated with the development of FECD. TCF4 encodes the E2-2 transcription factor but the link between the strong susceptibility locus and disease mechanism remains elusive. Here, we confirm a strong positive association between TCF4 single nucleotide polymorphism rs613872 and FECD in Polish patients (OR = 12.95, 95% CI: 8.63-19.42, χ (2) = 189.5, p < 0.0001). We show that TCF4 expression at the mRNA level in corneal endothelium (n = 63) does not differ significantly between individuals with a particular TCF4 genotype. It is also not altered in FECD patients as compared to control samples. The data suggest that changes in the transcript level containing constitutive TCF4 exon encoding the amino-terminal part of the protein seem not to contribute to disease pathogenesis. However, considering the strong association of TCF4 allelic variants with FECD, genotyping of TCF4 risk alleles may be important in the clinical practice.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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