Flavonoid Fraction of Citrus Reticulata Juice Reduces Proliferation and Migration of Anaplastic Thyroid Carcinoma Cells.

Effects of flavonoids extracted fromCitrus reticulata(mandarin) juice on proliferation and migration of 3 human anaplastic thyroid carcinoma (ATC) cell lines were evaluated. Flavonoid components of Mandarin juice extract (MJe) were analyzed by uHPLC. Proliferation of CAL-62, C-643, and 8505C cells,...

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Publicado en:Nutrition & Cancer Vol. 67; no. 7; pp. 1183 - 1191
Autores principales: Celano, Marilena, Maggisano, Valentina, De Rose, Roberta Francesca, Bulotta, Stefania, Maiuolo, Jessica, Navarra, Michele, Russo, Diego
Formato: research tables/charts Journal Article
Publicado: Taylor & Francis Ltd Oct2015
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Oct2015
      vid: 67
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      pub: Taylor & Francis Ltd
      place: Philadelphia, Pennsylvania
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        10.1080/01635581.2015.1073760
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        atl: Flavonoid Fraction of Citrus Reticulata Juice Reduces Proliferation and Migration of Anaplastic Thyroid Carcinoma Cells.
      aug:
        au:
          Celano, Marilena
          Maggisano, Valentina
          De Rose, Roberta Francesca
          Bulotta, Stefania
          Maiuolo, Jessica
          Navarra, Michele
          Russo, Diego
        affil: Department of Health Sciences, University of Catanzaro "Magna Græcia", Campus Universitario "S. Venuta", Catanzaro, Italy
      sug:
        subj:
          Flavonoids Therapeutic Use
          Plants, Medicinal Therapeutic Use
          Fruit Juices
          Thyroid Neoplasms Prevention and Control
          Nutrition
          Plant Extracts
          Human
          Cell Cycle
          Cell Physiology
          Proteins Physiology
          Blotting, Western
          Data Analysis
          Descriptive Statistics
          Post Hoc Analysis
          Funding Source
      ab: Effects of flavonoids extracted fromCitrus reticulata(mandarin) juice on proliferation and migration of 3 human anaplastic thyroid carcinoma (ATC) cell lines were evaluated. Flavonoid components of Mandarin juice extract (MJe) were analyzed by uHPLC. Proliferation of CAL-62, C-643, and 8505C cells, measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, was significantly reduced by MJe in a concentration- and time-dependent way, with maximal effect elicited at 0.5 mg/ml concentration after 48 h. Cytofluorimetric analysis showed a block in the G2/M phase of the cell cycle, accompanied by low cell mortality owed to autophagic death. The extract caused also a reduction of cell migration, associated with decreased activity of the metalloproteinase MMP-2. These findings demonstrate that the flavonoid fraction of mandarin juice exertsin vitroantiproliferative effects on ATC cells, associated with a reduction of migration, suggesting for such a functional food a potential use as adjuvant in the treatment of thyroid cancer.
      pubtype: Academic Journal
      doctype:
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        Journal Article
      ougenre: Article
    language: English
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