Inhibitory Effects of Isorhamnetin on the Invasion of Human Breast Carcinoma Cells by Downregulating the Expression and Activity of Matrix Metalloproteinase-2/9.
Matrix metalloproteinases (MMPs) play an active role in facilitating the invasion of cancer cells with excessive extracellular matrix (ECM) degradation. In the present study, we investigated the antiinvasive effects of isorhamnetin, a naturally occurring flavonoid, on MDA-MB-231 human breast carcino...
| Published in: | Nutrition & Cancer Vol. 67; no. 7; pp. 1191 - 1201 |
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| Main Authors: | , , , , , , , , |
| Format: | equations & formulas research tables/charts Journal Article |
| Published: |
Taylor & Francis Ltd
Oct2015
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=110072103&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 110072103 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01635581 7MS jtl: Nutrition & Cancer issn: 01635581 maglogo: N pubinfo: dt: Oct2015 vid: 67 iid: 7 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 110072103 110072103 110072103 10.1080/01635581.2015.1073763 NLM26359917 110072103 ppf: 1191 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Inhibitory Effects of Isorhamnetin on the Invasion of Human Breast Carcinoma Cells by Downregulating the Expression and Activity of Matrix Metalloproteinase-2/9. aug: au: Li, Chenglin Yang, Dan Zhao, Yuanwei Qiu, Yu Cao, Xin Yu, Yanyan Guo, Hao Gu, Xiaoke Yin, Xiaoxing affil: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Xuzhou Medical College, Xuzhou, People's Republic of China sug: subj: Breast Neoplasms Physiopathology Gene Expression Metalloproteins Nutrition Human Breast Neoplasms Prevention and Control Female Flavonoids Therapeutic Use Electrophoresis Neoplasm Metastasis Cell Culture Techniques Biological Assay Wound Healing Blotting, Western Polymerase Chain Reaction Enzyme-Linked Immunosorbent Assay Data Analysis Descriptive Statistics Funding Source Female ab: Matrix metalloproteinases (MMPs) play an active role in facilitating the invasion of cancer cells with excessive extracellular matrix (ECM) degradation. In the present study, we investigated the antiinvasive effects of isorhamnetin, a naturally occurring flavonoid, on MDA-MB-231 human breast carcinoma cells. The results indicated that isorhamnetin significantly inhibited the adhesion, migration, and invasion of the cells in vitro. Moreover, isorhamnetin suppressed the activity and expression of MMP-2 and MMP-9, which were determined by gelatin zymography, real-time PCR, and Western blot analysis, respectively. Besides, isorhamnetin had little effect on the secretion of urokinase plasminogen activator. Further elucidation of the mechanism revealed that isorhamnetin exerted an inhibitory effect on the phosphorylation of p38 and STAT3, although it had no effect on ERK1/2 and JNK. Taken together, these data demonstrated that isorhamnetin could significantly inhibit the invasion of MDA-MB-231 cells by downregulating the expression and activity of MMP-2 and MMP-9, which was potentially associated with the suppression of p38 MAPK and STAT3. Therefore, the findings provide new evidence for the anti-cancer activity of isorhamnetin. pubtype: Academic Journal doctype: equations & formulas research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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