The presence of high-risk human papillomavirus (HPV) E6/E7 mRNA transcripts in a subset of sinonasal carcinomas is evidence of involvement of HPV in its etiopathogenesis.
The aim of the study was to investigate prevalence of high-risk human papillomavirus (HR-HPV) infection in sinonasal carcinomas by immunohistochemistry, in situ hybridization, and polymerase chain reaction, detecting p16(INK4a) protein (p16) expression and presence of both HPV DNA and HPV E6/E7 mess...
| Publicado en: | Virchows Archiv: European Journal of Pathology Vol. 467; no. 4; pp. 405 - 416 |
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| Autores principales: | , , , , , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Oct2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=110401223&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 110401223 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09456317 O1Z jtl: Virchows Archiv: European Journal of Pathology issn: 09456317 maglogo: N pubinfo: dt: Oct2015 vid: 467 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 110401223 110401223 NLM26229021 110401223 10.1007/s00428-015-1812-x NLM26229021 110401223 ppf: 405 ppct: 11 formats: fmt: @attributes: type: P tig: atl: The presence of high-risk human papillomavirus (HPV) E6/E7 mRNA transcripts in a subset of sinonasal carcinomas is evidence of involvement of HPV in its etiopathogenesis. aug: au: Laco, Jan Sieglová, Kateřina Vošmiková, Hana Dundr, Pavel Němejcová, Kristýna Michálek, Jaroslav Čelakovský, Petr Chrobok, Viktor Mottl, Radovan Mottlová, Alena Tuček, Luboš Slezák, Radovan Chmelařová, Marcela Sirák, Igor Vošmik, Milan Ryška, Aleš Sieglová, Kateřina Vošmiková, Hana Němejcová, Kristýna Michálek, Jaroslav affil: The Fingerland Department of Pathology, Charles University in Prague, Faculty of Medicine and University Hospital in Hradec Kralove, Sokolska 581 500 05 Hradec Kralove Czech Republic sug: subj: Carcinoma, Squamous Cell Papillomaviruses RNA Analysis Head and Neck Neoplasms Paranasal Sinus Neoplasms Head and Neck Neoplasms Mortality Head and Neck Neoplasms Pathology Female Proteins Analysis Proteins Carcinoma, Squamous Cell Pathology Reverse Transcriptase Polymerase Chain Reaction Aged, 80 and Over RNA Adult Carcinoma, Squamous Cell Mortality Paranasal Sinus Neoplasms Mortality Middle Age DNA Analysis Male Aged Paranasal Sinus Neoplasms Pathology Immunohistochemistry Human Aged, 80 & over Adult: 19-44 years Middle Aged: 45-64 years Aged: 65+ years Female Male ab: The aim of the study was to investigate prevalence of high-risk human papillomavirus (HR-HPV) infection in sinonasal carcinomas by immunohistochemistry, in situ hybridization, and polymerase chain reaction, detecting p16(INK4a) protein (p16) expression and presence of both HPV DNA and HPV E6/E7 messenger RNA (mRNA). The study comprised 47 males and 26 females, aged 23-83 years (median 62 years), mostly (67 %) with a squamous cell carcinoma (SCC). Of the tumors, 53 % arose in the nasal cavity, 42 % in the maxillary sinus, and 5 % in the ethmoid complex. The follow-up period ranged 1-241 months (median 19 months). HPV16, HPV18, or HPV35 were detected in 18/73 (25 %) tumors, 17 SCCs, and 1 small cell neuroendocrine carcinoma. There was a strong correlation between results of HPV detection methods and p16 expression (p < 0.005). HPV-positive SCCs occurred more frequently in smokers (p = 0.04) and were more frequently p16-positive (p < 0.0001) and nonkeratinizing (p = 0.02), the latter occurring more commonly in nasal cavity (p = 0.025). Median survival for HPV-positive SCC patients was 30 months, while for HPV-negative SCC patients was 14 months (p = 0.23). In summary, we confirm that HR-HPV is actively involved in the etiopathogenesis of a significant subset of sinonasal SCCs. p16 may be used as a reliable surrogate marker for determination of HPV status also in sinonasal SCCs. Although we observed a trend toward better overall survival in HPV-positive SCCs, the prognostic impact of HPV status in sinonasal carcinomas needs to be elucidated by further studies. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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