Robust estimation of the motile cilia beating frequency.
The estimation of the cilia beating frequency (CBF) is of great interest in understanding how the CBF modulates liquid fluxes and how it is controlled by the ciliated cell intra- and/or extracellular medium composition in physiological processes. Motion artifacts and camera defaults may hinder the c...
| Publicado en: | Medical & Biological Engineering & Computing Vol. 53; no. 10; pp. 1025 - 1036 |
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| Autores principales: | , , |
| Formato: | equations & formulas pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Oct2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=110401383&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 110401383 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01400118 PO0 jtl: Medical & Biological Engineering & Computing issn: 01400118 maglogo: N pubinfo: dt: Oct2015 vid: 53 iid: 10 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 110401383 110401383 NLM26215519 110401383 10.1007/s11517-015-1345-0 NLM26215519 110401383 ppf: 1025 ppct: 11 formats: fmt: @attributes: type: P tig: atl: Robust estimation of the motile cilia beating frequency. aug: au: Meste, O. Brau, F. Guyon, A. affil: Lab I3S, UMR 7271, CNRS, University of Nice-Sophia Antipolis, Sophia Antipolis France sug: subj: Cytological Techniques Methods Cells Mice Animal Studies Artifacts Male Signal Processing, Computer Assisted Male ab: The estimation of the cilia beating frequency (CBF) is of great interest in understanding how the CBF modulates liquid fluxes and how it is controlled by the ciliated cell intra- and/or extracellular medium composition in physiological processes. Motion artifacts and camera defaults may hinder the computation of the frequency variations during long-lasting experiments. We have developed a new analysis approach consisting of a preliminary corrective step (removal of a grid pattern on the image sequence and shift compensation), followed by a harmonic model of the observed cilia using a maximum likelihood estimator framework. It is shown that a more accurate estimation of the frequency can be obtained by averaging the squared Fourier transform of individual pixels followed by a particular summation over the different frequencies, namely the compressed spectrum. Robustness of the proposed method over traditional approaches is shown by several examples and simulations. The method is then applied to images of samples containing ciliated ependymal cells located in the third cerebral ventricle of mouse brains, showing that even small variations in CBF in response to changes in the amount of oxygenation, pH or glucose were clearly visible in the computed frequencies. As a conclusion, this method reveals a fine metabolic tuning of the cilia beating in ependimocytes lining the third cerebral ventricle. Such regulations are likely to participate in homeostatic mechanisms regulating CSF movements and brain energy supply. pubtype: Academic Journal doctype: equations & formulas pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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