Frequency of γδ T Cells and Invariant Natural Killer T Cells in Helicobacter Pylori-infected Patients with Peptic Ulcer and Gastric Cancer.

To clarify the effect of γδ T cells and invariant Natural Killer T (iNKT) cells in pathophysiology of dyspeptic disorders, number of these two cells in patients with non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), and gastric cancer (GC) were compared.Patients with dyspepsia were divided into...

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Publicado en:Iranian Journal of Allergy, Asthma & Immunology Vol. 14; no. 5; pp. 493 - 502
Autores principales: Shadman, Mojtaba, Rajabian, Zeinab, Ajami, Abolghasem, Hussein-Nattaj, Hadi, Rafiei, Alireza, Hosseini, Vahid, Taghvaei, Tarang, Abbasi, Ali, Tehrani, Mohsen
Formato: pictorial research tables/charts Journal Article
Publicado: Tehran University of Medical Sciences Oct2015
Acceso en línea:Ver este registro en EBSCOhost
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        atl: Frequency of γδ T Cells and Invariant Natural Killer T Cells in Helicobacter Pylori-infected Patients with Peptic Ulcer and Gastric Cancer.
      aug:
        au:
          Shadman, Mojtaba
          Rajabian, Zeinab
          Ajami, Abolghasem
          Hussein-Nattaj, Hadi
          Rafiei, Alireza
          Hosseini, Vahid
          Taghvaei, Tarang
          Abbasi, Ali
          Tehrani, Mohsen
        affil: Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
      sug:
        subj:
          Peptic Ulcer Immunology
          Adenocarcinoma Immunology
          T Lymphocytes Immunology
          Receptors, Cell Surface Metabolism
          Dyspepsia Immunology
          Helicobacter Infections Immunology
          Stomach Neoplasms Immunology
          Stomach Neoplasms Pathology
          T Lymphocytes
          Receptors, Cell Surface Immunology
          Helicobacter Pylori
          Dyspepsia Pathology
          Stomach Neoplasms Metabolism
          Endoscopy, Digestive System
          Adult
          Immunohistochemistry
          Middle Age
          Dyspepsia Metabolism
          Female
          Male
          Stomach Immunology
          Flow Cytometry
          Helicobacter Infections Metabolism
          Helicobacter Infections Pathology
          Peptic Ulcer Pathology
          Peptic Ulcer Metabolism
          Aged
          Adenocarcinoma Pathology
          Biopsy
          Adenocarcinoma Metabolism
          Stomach Metabolism
          Stomach Pathology
          Human
          Adult: 19-44 years
          Middle Aged: 45-64 years
          Aged: 65+ years
          Female
          Male
      ab: To clarify the effect of γδ T cells and invariant Natural Killer T (iNKT) cells in pathophysiology of dyspeptic disorders, number of these two cells in patients with non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), and gastric cancer (GC) were compared.Patients with dyspepsia were divided into three groups of NUD, PUD, and GC according to their endoscopic and histopathological examinations. Helicobacter pylori infection was diagnosed by rapid urease test and histopathology. The number of peripheral blood CD3+TCRγδ(+) T cells and CD3+Va24Ja18+ iNKT cells were determined by flow cytometry. Immunohistochemistry (IHC) was also used for identifying the TCRγδ+ cells.Forty two patients with NUD (31.6%), 44 with PUD (33.1%), and 47 with GC (35.3%) were included in the study. The frequency of CD3+TCRγδ(+) T cells in peripheral blood of patients with GC (2.71±0.25) was significantly lower than that in NUD (3.97±0.32, p<0.05) and PUD groups (3.87±0.32, p<0.05). However, there was no significant difference in CD3+TCRγδ(+) T cell percentage between the NUD and PUD groups. The frequency of TCRγδ(+) lymphocytes was significantly lower in tissue samples from patients with GC (4.81±0.53) than in NUD (11.09±1.09, p<0.0001) and PUD groups (11.11±1.01, p<0.0001). Also, we could not find any significant difference in the percentage of mucosal TCRγδ+ cells between the NUD and PUD groups. The results showed no significant difference in iNKT cells percentage among the three groups of patients.The results suggest that decreasing number of γδ T cells may be related to development and progression of gastric cancer.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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