Antral gastric permeability to antigens in mice is altered by infection with Helicobacter felis.
Objective: Gastric inflammation is observed not only during Helicobacter pylori infection but also after eradication of the bacterium. The hypothesis that an altered gastric permeability could be involved was tested using a model of mice infected with Helicobacter felis.Design: The antral and corpus...
| Publicado en: | European Journal of Gastroenterology & Hepatology Vol. 11; no. 12; pp. 1371 - 1378 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Lippincott Williams & Wilkins
Dec1999
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=110639809&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 110639809 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0954691X 8CE jtl: European Journal of Gastroenterology & Hepatology issn: 0954691X maglogo: N pubinfo: dt: Dec1999 vid: 11 iid: 12 pid: 5086 pub: Lippincott Williams & Wilkins place: Baltimore, Maryland artinfo: ui: 110639809 110639809 NLM10654797 110639809 10.1097/00042737-199912000-00006 NLM10654797 110639809 ppf: 1371 ppct: 7 formats: tig: atl: Antral gastric permeability to antigens in mice is altered by infection with Helicobacter felis. aug: au: Matysiak-Budnik, Tamara Hashimoto, Kei heyman, Martine de mascarel, Antoine Desjeux, Jehan-Francois Mégraud, Francis Matysiak-Budnik, T Hashimoto, K Heyman, M de Mascarel, A Desjeux, J F Mégraud, F affil: INSERM E9925, Faculté Necker, 156 rue de vaugirard, 75730 Paris, France sug: subj: Gastric Mucosa Metabolism Helicobacter Infections Metabolism Antigens Metabolism Biological Transport Helicobacter Infections Microbiology Animal Studies Permeability Helicobacter Gastritis Etiology Pyloric Antrum Metabolism Mice Pyloric Antrum Microbiology Urease Metabolism Gastric Mucosa Microbiology Gastric Mucosa Validation Studies Comparative Studies Evaluation Research Multicenter Studies ab: Objective: Gastric inflammation is observed not only during Helicobacter pylori infection but also after eradication of the bacterium. The hypothesis that an altered gastric permeability could be involved was tested using a model of mice infected with Helicobacter felis.Design: The antral and corpus gastric permeability during infection and after eradication of bacteria was studied.Methods: Gastric fragments from the antrum and corpus of healthy mice, mice infected with H. felis, or mice after bacterial eradication, were mounted in Ussing chambers, and fluxes of sodium (JNa), mannitol (JMan) and horseradish peroxidase (HRP) under intact (JHRPi) and degraded (JD) form were measured.Results: In healthy mice, JNa, JMan, JHRPi and JD, respectively, were greater in the antrum (6.5 +/- 0.5 microEq/h.cm2; 0.137 +/- 0.016 micromol/h.cm2; 30.4 +/- 7.4 ng/h.cm2 and 852 +/- 173 ng/h.cm2) than in the corpus (5.0 +/- 0.3 microEq/h.cm2; 0.085 micro 0.013 micromol/h.cm2; 9.5 +/- 2.8 ng/h.cm2 and 434 +/- 139 ng/h.cm2). In H. felis-infected mice, HRP fluxes in the antrum were increased (JHRPi = 182 +/- 86, JD = 948 +/- 94 ng/h.cm2) as compared to controls (JHRPi = 10.3 +/- 2.6, JD = 458 +/- 98 ng/h.cm2). Bacterial eradication led to the reduction of intact (JHRPi = 53 +/- 26 ng/h.cm) but not of degraded (JD = 844 +/- 213 ng/h.cm) HRP fluxes. After eradication, degraded HRP fluxes returned to normal in mice without inflammation (JD = 558 +/- 36 ng/h.cm2) but not in those with persistent inflammation (JD = 987 +/- 310 ng/h.cm2).Conclusions: The results suggest that during H. felis infection, bacterial colonization and inflammation lead to an increased gastric permeability along the direct and degradative pathways, respectively. Such an increased antigenic load could contribute to the perpetuation of gastric inflammation after bacterial eradication, and possibly to food protein sensitization. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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