Seroprevalence of eight Helicobacter pylori antigens among 182 patients with peptic ulcer, MALT gastric lymphoma or non-ulcer dyspepsia. Higher rate of seroreactivity against CagA and 35-kDa antigens in patients with peptic ulcer originating from Europe and Africa.
Background: It has been suggested that Helicobacter pylori may induce more or less severe gastroduodenal disease according to the strain virulence.Design: We used Western blot to determine antigenic profiles associated with duodenal or gastric ulcer disease, MALT lymphoma and non-ulcer dyspepsia, an...
| Publicado en: | European Journal of Gastroenterology & Hepatology Vol. 11; no. 7; pp. 721 - 727 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Lippincott Williams & Wilkins
Jul1999
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=110640252&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 110640252 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0954691X 8CE jtl: European Journal of Gastroenterology & Hepatology issn: 0954691X maglogo: N pubinfo: dt: Jul1999 vid: 11 iid: 7 pid: 5086 pub: Lippincott Williams & Wilkins place: Baltimore, Maryland artinfo: ui: 110640252 110640252 NLM10445790 110640252 10.1097/00042737-199907000-00007 NLM10445790 110640252 ppf: 721 ppct: 6 formats: tig: atl: Seroprevalence of eight Helicobacter pylori antigens among 182 patients with peptic ulcer, MALT gastric lymphoma or non-ulcer dyspepsia. Higher rate of seroreactivity against CagA and 35-kDa antigens in patients with peptic ulcer originating from Europe and Africa. aug: au: Lamarque, Dominique Gilbert, Thierry Roudot-Thoraval, Françoise Deforges, Lionel Chaumette, Marie Thérèse Delchier, Jean Charles Lamarque, D Gilbert, T Roudot-Thoraval, F Deforges, L Chaumette, M T Delchier, J C affil: Service d'Hèpatologie et de Gastroentérologie, bService de Santé Publique, cService de Bactériologie et de Virologie, dDepartement d'Anatomie Pathologique, Centre Hospitalier Universitaire Henri Mondor, 51 Avenue du Maréchal De Lattre de Tassigny, F-94010 Créteil, France sug: subj: Helicobacter Pylori Immunology Peptic Ulcer Immunology Dyspepsia Immunology Helicobacter Infections Immunology Lymphoma, B-Cell Immunology Antigens, Bacterial Blood Stomach Neoplasms Immunology Stomach Neoplasms Microbiology Peptic Ulcer Epidemiology Peptic Ulcer Microbiology Europe Helicobacter Infections Epidemiology Dyspepsia Microbiology Genes Duodenal Ulcer Epidemiology Antibodies, Bacterial Analysis Heat-Shock Proteins Immunology Blotting, Western Nonparametric Statistics Africa Duodenal Ulcer Microbiology Epidemiological Research Lymphoma, B-Cell Microbiology Duodenal Ulcer Immunology Oxidoreductases Bacterial Proteins Immunology Human ab: Background: It has been suggested that Helicobacter pylori may induce more or less severe gastroduodenal disease according to the strain virulence.Design: We used Western blot to determine antigenic profiles associated with duodenal or gastric ulcer disease, MALT lymphoma and non-ulcer dyspepsia, and to identify geographical differences.Methods: One hundred and eighty-two consecutive patients with H. pylori infection were studied. H. pylori infection was diagnosed by a rapid urease test or histological examination of gastric biopsy samples. Bacterial density and gastritis were assessed histologically by using the Sydney scoring system. Western blot was used to identify antibodies against eight antigens (CagA, VacA, urease A, heat shock protein B, and 19.5, 26.5, 30 and 35 kDa). Patients were questioned on their smoking habits and place of birth and childhood.Results: There were 73 patients with duodenal ulcer, 30 with gastric ulcer, eight with erosive duodenitis, 17 with gastric MALT lymphoma and 54 with non-ulcer dyspepsia. Most (>85%) were seropositive for the heat shock protein B and 26.5-kDa antigens. The prevalence of the other antigens ranged from 45% (VacA) to 68% (urease B). The seroprevalence of CagA antigen was significantly higher (P < 0.01) in cases of gastroduodenal ulcer (84%) than non-ulcer dyspepsia (37%). Similarly, 35-kDa antigen reactivity was more frequent (P < 0.05) in duodenal ulcer patients (75%) than in those with non-ulcer dyspepsia (50%). The antigenic profiles associated with MALT gastric lymphoma and non-ulcer dyspepsia were similar. Multivariate analysis showed that only gastroduodenal ulcer was significantly associated with CagA. Gastroduodenal ulcer and a childhood spent in Africa were both associated with 35-kDa and combined CagA-35-kDa reactivity.Conclusions: This study confirms the strong seroprevalence of H. pylori CagA antigen and shows a high prevalence of the 35-kDa antigen in patients with gastroduodenal ulcer, especially those raised in Africa. There was no difference in the serological pattern between patients with non-ulcer dyspepsia and those with MALT lymphoma. Tests for antibodies to the CagA-35-kDa antigen combination might be used to select H. pylori-infected dyspeptic patients warranting treatment. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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