Detection of Helicobacter pylori cagA gene by polymerase chain reaction in faecal samples.
Objective: The polymerase chain reaction (PCR) has been extensively and successfully used to detect Helicobacter pylori in gastric juice and gastric biopsies. In contrast, the results obtained using faeces as biological samples for PCR are rather conflicting. This may be due to the presence of faeca...
| Publicado en: | European Journal of Gastroenterology & Hepatology Vol. 11; no. 3; pp. 251 - 257 |
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| Autores principales: | , , , , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Lippincott Williams & Wilkins
Mar1999
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=110640339&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 110640339 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0954691X 8CE jtl: European Journal of Gastroenterology & Hepatology issn: 0954691X maglogo: N pubinfo: dt: Mar1999 vid: 11 iid: 3 pid: 5086 pub: Lippincott Williams & Wilkins place: Baltimore, Maryland artinfo: ui: 110640339 110640339 NLM10333197 110640339 10.1097/00042737-199903000-00008 NLM10333197 110640339 ppf: 251 ppct: 6 formats: tig: atl: Detection of Helicobacter pylori cagA gene by polymerase chain reaction in faecal samples. aug: au: Russo, Francesco Notarnicola, Maria Matteo, Giovanni Di Leoci, Claudio Caruso, Maria Lucia Pirrelli, Michele Caradonna, Maurizio Morandi, Luca Leo, Alfredo Di Russo, F Notarnicola, M Di Matteo, G Leoci, C Caruso, M L Pirrelli, M Caradonna, M Morandi, L Di Leo, A affil: Laboratory of Biochemistry, bEndoscopy Unit, and cDepartment of Pathology, IRCCS Scientific Institute for Digestive Diseases 'S. de Bellis', Castellana G., Bari, Italy, dEurospital SpA, Trieste, Italy, and eICGEB, Trieste, Italy sug: subj: Bacterial Proteins Antigens, Bacterial Feces Microbiology Helicobacter Pylori Adult Middle Age Polymerase Chain Reaction Aged Stomach Pathology Polysaccharides, Bacterial Duodenal Ulcer Pathology Gastritis Pathology Urease Male DNA Probes Gastric Mucosa Microbiology Female Duodenal Ulcer Microbiology Gastroscopy Human Pyloric Antrum Microbiology Dyes Gastritis Microbiology Stomach Microbiology Pyloric Antrum Pathology Gastric Mucosa Pathology Validation Studies Comparative Studies Evaluation Research Multicenter Studies Adult: 19-44 years Middle Aged: 45-64 years Aged: 65+ years Male Female ab: Objective: The polymerase chain reaction (PCR) has been extensively and successfully used to detect Helicobacter pylori in gastric juice and gastric biopsies. In contrast, the results obtained using faeces as biological samples for PCR are rather conflicting. This may be due to the presence of faecal inhibitory compounds (polysaccharides) which can inhibit the amplification reaction. The aim of this study was to characterize the H. pylori genotype in faecal samples by using specific primers for the cagA gene. To overcome the problem of contamination by polysaccharides, we used a filter-based extraction technique already applied in a previous study.Methods: Antral and body biopsies were obtained from 30 symptomatic patients undergoing upper endoscopy. PCR was used to detect the presence of H. pylori organisms in faecal samples by using primers selected for the urease gene A. In addition, H. pylori organisms were characterized both in faecal samples and paraffin-embedded biopsies by PCR with specific primers for the cagA gene.Results: All patients showed a positive CLO test (rapid urease test) and evidence of H. pylori by Warthin-Starry stain. PCR detected the urease A gene in the faecal samples of all patients. The cagA gene was detected in the faecal and biopsy samples of 18 subjects (60%). Duodenal ulcer and/or antral erosions were observed in 15 of the 18 cagA-positive patients (83.3%) and in five of the 12 cagA-negative patients (41.7%). Endoscopic features of normal mucosa or gastritis were observed in three cagA-positive patients (16.7%) and in seven cagA-negative patients (56.3%). cagA-positive status was found to be significantly related to the endoscopic features of duodenal ulceration and/or antral erosions.Conclusions: Our findings prove that faeces are suitable samples for the detection of cagA status. Moreover, they confirm the existence of a significant relationship between cagA-positive status and duodenal ulcer and/or antral erosions. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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