Qualitative and semi-quantitative value of a modified 13C-urea breath test for identification of Helicobacter pylori infection.
Objective: The 13C-urea breath test (13C-UBT) is the method of choice in evaluating the success of therapy for eradication of Helicobacter pylori infection. For reasons of cost efficiency and practicability, urea dose and measurement duration have been reduced and the DOB (delta over baseline) cutof...
| Publicado en: | European Journal of Gastroenterology & Hepatology Vol. 9; no. 11; pp. 1085 - 1090 |
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| Autores principales: | , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Lippincott Williams & Wilkins
Nov1997
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=110640925&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 110640925 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0954691X 8CE jtl: European Journal of Gastroenterology & Hepatology issn: 0954691X maglogo: N pubinfo: dt: Nov1997 vid: 9 iid: 11 pid: 5086 pub: Lippincott Williams & Wilkins place: Baltimore, Maryland artinfo: ui: 110640925 110640925 NLM9431899 110640925 10.1097/00042737-199711000-00011 NLM9431899 110640925 ppf: 1085 ppct: 5 formats: tig: atl: Qualitative and semi-quantitative value of a modified 13C-urea breath test for identification of Helicobacter pylori infection. aug: au: Ellenrieder, Volker Glasbrenner, Bernd Stoffels, Christian Weiler, Stefanie Bode, Günter Möller, Peter Adler, Guido Ellenrieder, V Glasbrenner, B Stoffels, C Weiler, S Bode, G Möller, P Adler, G affil: From the Departments of 1Gastroenterology and 2Pathology, University of Ulm, Ulm, Germany. sug: subj: Urea Metabolism Helicobacter Pylori Metabolism Helicobacter Infections Diagnosis Breath Tests Methods Isotopes Middle Age Bacteriological Techniques Pyloric Antrum Microbiology Stomach Microbiology Adult Male Female Predictive Value of Tests Urease Metabolism Aged Human Histocytochemistry Prospective Studies Adolescence Helicobacter Pylori Sensitivity and Specificity Validation Studies Comparative Studies Evaluation Research Multicenter Studies Middle Aged: 45-64 years Adult: 19-44 years Aged: 65+ years Adolescent: 13-18 years Male Female ab: Objective: The 13C-urea breath test (13C-UBT) is the method of choice in evaluating the success of therapy for eradication of Helicobacter pylori infection. For reasons of cost efficiency and practicability, urea dose and measurement duration have been reduced and the DOB (delta over baseline) cutoff level with the highest predictive value determined. Further, the efficacy of the modified 13C-UBT as a semi-quantitative test method was evaluated by comparison with histologically determined bacterial infiltration.Methods: In a prospective study, a modified 13C-UBT with reduced urea dose (75 mg) and shortened measurement duration (30 min) was administered to 145 patients. The DOB cutoff with the highest predictive value was determined using relative operating characteristic analysis. Reference methods included histology, bacterial culture and the rapid urease test. The DOB value was compared with the histologically determined grade of bacterial infiltration and the correlation evaluated using the Spearman ranking method.Results: Reduction of the DOB cutoff level from 5.0 per thousand to 3.5 per thousand led to significant improvement in sensitivity (78.9% vs. 91.2%) and accuracy (88.6% vs. 90.2%) of the 13C-UBT. Only five of 57 infected patients were incorrectly reported as non-infected after modification of the DOB level. In two of three patients in whom histological findings were negative but the 13C-urease test positive, bacterial growth was observed at culture. The DOB level correlated significantly with histological grade of bacterial infection.Conclusion: The modified 13C-UBT proved to be a sensitive, practicable and cost-effective method for detecting H. pylori infection and permits a semi-quantitative estimation of bacterial infiltration. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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