Helicobacter pylori-human polymorphonuclear leucocyte interaction in the presence of ammonia.
Objective: To determine if the ammonia produced by Helicobacter pylori affects the phagocytic ability of human polymorphonuclear leucocytes as measured by the oxidative burst.Methods: Interactions between opsonized urease-positive and -negative strains of H. pylori with polymorphonuclear leucocytes...
| Publicado en: | European Journal of Gastroenterology & Hepatology Vol. 9; no. 5; pp. 457 - 462 |
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| Autores principales: | , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Lippincott Williams & Wilkins
May1997
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=110641027&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 110641027 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0954691X 8CE jtl: European Journal of Gastroenterology & Hepatology issn: 0954691X maglogo: N pubinfo: dt: May1997 vid: 9 iid: 5 pid: 5086 pub: Lippincott Williams & Wilkins place: Baltimore, Maryland artinfo: ui: 110641027 110641027 NLM9187877 110641027 10.1097/00042737-199705000-00009 NLM9187877 110641027 ppf: 457 ppct: 5 formats: tig: atl: Helicobacter pylori-human polymorphonuclear leucocyte interaction in the presence of ammonia. aug: au: Mayo, Kathryn Held, Maria Wadström, Torkel Mégraud, Francis Mayo, K Held, M Wadström, T Mégraud, F affil: From the 1Laboratoire de Bactériologie-Enfants, Hopital Pellegrin, Bordeaux, France and 2Department of Medical Microbiology, University of Lund, Sweden. sug: subj: Cell Physiology Drug Effects Phagocytosis Drug Effects Ammonia Pharmacodynamics Neutrophils Physiology Helicobacter Pylori Physiology Helicobacter Infections Microbiology Stomach Diseases Pathology Dose-Response Relationship, Drug Urease Metabolism Gastric Mucosa Metabolism Neutrophils Drug Effects Urea Pharmacodynamics Oxygen Consumption Helicobacter Pylori Helicobacter Infections Pathology Hydrogen-Ion Concentration Biopsy Gastric Mucosa Microbiology Stomach Diseases Etiology Human Luminescent Measurements Validation Studies Comparative Studies Evaluation Research Multicenter Studies ab: Objective: To determine if the ammonia produced by Helicobacter pylori affects the phagocytic ability of human polymorphonuclear leucocytes as measured by the oxidative burst.Methods: Interactions between opsonized urease-positive and -negative strains of H. pylori with polymorphonuclear leucocytes were studied in two series of experiments. In the first series of experiments, concentrations from 0 to 50 mM of NH4Cl were added to polymorphonuclear leucocytes. In the second series of experiments, bacteria were pre-incubated for 1 h with urea (0 to 50 mM) before addition of phagocytes. Luminol-dependent chemiluminescence was measured every 5 min over a 50-min period. The pH was verified in each treatment.Results: Inhibition of chemiluminescence, increasing with concentration, was noted in all treatments when NH4Cl was added. When urea was added to urease-positive strains, chemiluminescence was significantly reduced when compared to the urease-negative strain and the zymosan control. This effect could not be attributed to a change in pH in the experiments using NH4Cl or urea at a concentration of 5 mM and 10 mM.Conclusion: Ammonia generated by H. pylori may contribute to the decreased activity of polymorphonuclear leucocytes in vivo. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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