Superoxide dismutase activity in duodenal ulcer patients.
Objective: To investigate whether duodenal ulcer (DU) is associated with increased free radical generation at the site of ulceration and to attempt, indirectly, to confirm the hypothesis by determining activity of free radical scavengers such as superoxide dismutase (SOD).Study Design: Prospective s...
| Publicado en: | European Journal of Gastroenterology & Hepatology Vol. 8; no. 12; pp. 1151 - 1156 |
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| Autores principales: | , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Lippincott Williams & Wilkins
Dec1996
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=110687298&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 110687298 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0954691X 8CE jtl: European Journal of Gastroenterology & Hepatology issn: 0954691X maglogo: N pubinfo: dt: Dec1996 vid: 8 iid: 12 pid: 5086 pub: Lippincott Williams & Wilkins place: Baltimore, Maryland artinfo: ui: 110687298 110687298 NLM8980931 110687298 10.1097/00042737-199612000-00003 NLM8980931 110687298 ppf: 1151 ppct: 5 formats: tig: atl: Superoxide dismutase activity in duodenal ulcer patients. aug: au: Klinowski, Elieser Broide, Efrat Varsano, Rina Eshchar, Jerachmiel Scapa, Eitan Klinowski, E Broide, E Varsano, R Eshchar, J Scapa, E affil: From the Intensive Care Unit, Rehovot, Israel sug: subj: Reactive Oxygen Species Metabolism Duodenal Ulcer Superoxide Dismutase Metabolism Middle Age Duodenum Pathology Duodenal Ulcer Microbiology Gastric Mucosa Pathology Helicobacter Infections Diagnosis Male Helicobacter Pylori Biopsy Duodenal Ulcer Pathology Duodenum Gastric Mucosa Human Female Case Control Studies Validation Studies Comparative Studies Evaluation Research Multicenter Studies Middle Aged: 45-64 years Male Female ab: Objective: To investigate whether duodenal ulcer (DU) is associated with increased free radical generation at the site of ulceration and to attempt, indirectly, to confirm the hypothesis by determining activity of free radical scavengers such as superoxide dismutase (SOD).Study Design: Prospective study comparing SOD activity in biopsies taken from the ulcer edge, and antrum in DU patients before and after one month of treatment to SOD activity in biopsies from the duodenal bulb and antrum in a control population.Setting: Institute of Gastroenterology of a university hospital.Study Population: Twenty-five patients with DU. The diagnosis was confirmed by endoscopy.Measurements: SOD activity in biopsy specimen, red blood cells (RBC) and serum was measured using the cytochrome c method by spectrophotometry and expressed as U SOD/mg protein. Helicobacter pylori was diagnosed by the rapid urease test (CLO test) from antral biopsies.Results: SOD activity was markedly depleted in the ulcer edge (2.78 +/- 0.9 U SOD/mg protein) as compared to the same patients after one month of treatment (4.59 +/- 2.2 U SOD/mg protein) (P = 0.05) and to biopsies from the duodenal bulb in control population (6.7 +/- 2.4 U.SOD/mg protein) (P = 0.05). SOD activity in the antrum of both groups was similar. There was no difference in SOD activity in RBC and serum of both groups.Conclusion: Products of free radical reactions are implicated in the pathogenesis of DU disease. SOD, which is a key enzyme in gastric mucosal protection, is depleted significantly in the ulcer edge compared with controls and increases after healing. However, it is not clear whether this abnormality in oxygen free radical metabolism reflects, rather than causes, the condition which characterizes DU. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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