In Vivo Tracking of Streptococcal Infections of Subcutaneous Origin in a Murine Model.
Purpose: Generation of plasmin in vivo by Streptococcus pyogenes is thought to localize the active protease complexes to the pathogen surface to aid in tissue dissemination. Here, we chose to follow cutaneous streptococcal infections by the use of non-invasive bioluminescence imaging to determine if...
| Publicado en: | Molecular Imaging & Biology Vol. 17; no. 6; pp. 793 - 802 |
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| Autores principales: | , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Dec2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=110848922&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 110848922 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Dec2015 vid: 17 iid: 6 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 110848922 110848922 NLM25921659 110848922 10.1007/s11307-015-0856-2 NLM25921659 PMC4807872 [Available on 12/01/16] 110848922 ppf: 793 ppct: 9 formats: fmt: @attributes: type: P tig: atl: In Vivo Tracking of Streptococcal Infections of Subcutaneous Origin in a Murine Model. aug: au: Davis, Richard Eggleston, Heather Johnson, Frances Nahrendorf, Matthias Bock, Paul Peterson, Tiffany Panizzi, Peter Davis, Richard W 4th Bock, Paul E affil: Department of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, 4306 Walker Building Auburn 36849 USA sug: subj: Models, Biological Streptococcal Infections Microbiology Skin Microbiology Streptococcus Mice Animal Studies ab: Purpose: Generation of plasmin in vivo by Streptococcus pyogenes is thought to localize the active protease complexes to the pathogen surface to aid in tissue dissemination. Here, we chose to follow cutaneous streptococcal infections by the use of non-invasive bioluminescence imaging to determine if this pathogen can be followed by this approach and the extent of bacterial spread in the absence of canonical plasminogen activation by streptokinase.Procedures: Mice were injected subcutaneously with either bioluminescent strains of streptococci, namely Xen20 and Xen10 or S. pyogenes ALAB49. Bioluminescence imaging was performed daily and results were correlated with microbiological and histological analyses.Results: Comparative analysis of chronologic non-invasive datasets indicated that Xen20 did not disseminate from the initial infection site. Contrary to this, microbiological and histological analyses of Xen20 mice for total bacterial burden indicated sepsis and widespread pathogen involvement.Conclusions: The use of bioluminescence in microbe-based studies requires genomic and pathologic characterization to correlate imaging results with underlying pathology. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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