The expression and post-transcriptional regulation of FSTL1 transcripts in placental trophoblasts.
Introduction: Follistatin-like-1 (FSTL1) is a widely expressed secreted protein with diverse but poorly understood functions. Originally described as a pro-inflammatory molecule, it has recently been reported to play a role in signaling pathways that regulate development and homeostasis. Distinctive...
| Publicado en: | Placenta Vol. 36; no. 11; pp. 1231 - 1239 |
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| Autores principales: | , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
Nov2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=110855862&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 110855862 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01434004 NZZ jtl: Placenta issn: 01434004 maglogo: N pubinfo: dt: Nov2015 vid: 36 iid: 11 pid: 467 pub: Elsevier B.V. place: New York, New York artinfo: ui: 110855862 110855862 NLM26386648 110855862 10.1016/j.placenta.2015.09.005 NLM26386648 PMC4644452 [Available on 11/01/16] 110855862 ppf: 1231 ppct: 8 formats: tig: atl: The expression and post-transcriptional regulation of FSTL1 transcripts in placental trophoblasts. aug: au: Mouillet, Jean-Francois Mishima, Takuya Paffaro, Andrea Mollica do Amarante Parks, Tony W. Ziegler, Judy A. Chu, Tianjiao Sadovsky, Yoel affil: Magee-Womens Research Institute, University of Pittsburgh, PA, USA sug: subj: Blastocyst Metabolism Carrier Proteins Metabolism RNA Metabolism Cell Line RNA Anoxia Metabolism Funding Source Human ab: Introduction: Follistatin-like-1 (FSTL1) is a widely expressed secreted protein with diverse but poorly understood functions. Originally described as a pro-inflammatory molecule, it has recently been reported to play a role in signaling pathways that regulate development and homeostasis. Distinctively, FSTL1 harbors within its 3'-UTR the sequence encoding microRNA-198 (miR-198), shown to be inversely regulated relative to FSTL1 expression and to exhibit opposite actions on cellular processes such as cell migration. We sought to investigate the expression of FSTL1 and to assess its interplay with miR-198 in human trophoblasts.Methods: We used a combination of northern blot analyses, quantitative PCR, small RNA sequencing, western blot and immunohistochemistry to characterize FSTL1 and miR-198 expression in placental trophoblasts. We also used reporter assays to examine the post-transcriptional regulation of FSTL1 and assess its putative regulation by miR-198.Results: We detected the expression of FSTL1 transcript in both the human extravillous trophoblast line HTR-8/SVneo and in primary term human villous trophoblasts. We also found that the expression of FSTL1 was largely restricted to extravillous trophoblasts. Hypoxia enhanced the expression of FSTL1 protein in cultured primary villous trophoblasts. Interestingly, we did not detect any evidence for expression or function of mature miR-198 in human trophoblasts.Discussion: Our data indicate that placental FSTL1 is expressed particularly in extravillous trophoblasts. We also found no evidence for placental expression of miR-198, or for its regulation of FSTL1, implying that the post-transcriptional regulation of FSTL1 by miR-198 is tissue specific. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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