Antibacterial, antifungal and antioxidant activity of against pathogenic Olea africana yeast and nosocomial pathogens.
Background: Olea africana leaves are used by Bapedi people to treat different ailments. The use of these leaves is not validated, therefore the aim of this study is to validate antimicrobial properties of this plant. Methods: The ground leaves were extracted using solvents of varying polarity (hexan...
| Publicado en: | BMC Complementary & Alternative Medicine Vol. 15; pp. 1 - 10 |
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| Autores principales: | , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
BioMed Central
11/17/2015
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=111059206&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 111059206 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14726882 1CHF jtl: BMC Complementary & Alternative Medicine issn: 14726882 maglogo: N pubinfo: dt: 11/17/2015 vid: 15 pid: 24147 pub: BioMed Central artinfo: ui: 111059206 111059206 111059206 10.1186/s12906-015-0941-8 111059206 ppf: 1 ppct: 9 formats: tig: atl: Antibacterial, antifungal and antioxidant activity of against pathogenic Olea africana yeast and nosocomial pathogens. aug: au: Masoko, Peter Makgapeetja, David M. affil: Department of Biochemistry, Microbiology and Biotechnology, University of Limpopo, Private bag X1106, Sovenga 0727, South Africa sug: subj: Antibiotics Administration and Dosage Antifungal Agents Administration and Dosage Antioxidants Administration and Dosage Yeasts Drug Effects Plant Leaves Administration and Dosage Plant Leaves Analysis Bacteria Drug Effects Chromatography, Thin Layer Biological Assay Escherichia Coli Drug Effects Pseudomonas Drug Effects Enterococcus Faecium Drug Effects Staphylococcus Aureus Drug Effects Candida Albicans Drug Effects Cryptococcus Drug Effects Chemistry, Analytical Descriptive Statistics Funding Source ab: Background: Olea africana leaves are used by Bapedi people to treat different ailments. The use of these leaves is not validated, therefore the aim of this study is to validate antimicrobial properties of this plant. Methods: The ground leaves were extracted using solvents of varying polarity (hexane, chloroform, dichloromethane (DCM), ethyl acetate, acetone, ethanol, methanol, butanol and water). Thin layer chromatography (TLC) was used to analyse the chemical constituents of the extracts. The TLC plates were developed in three different solvent systems, namely, benzene/ethanol/ammonium solution (BEA), chloroform/ethyl acetate/formic acid (CEF) and ethyl acetate/methanol/water (EMW). The micro-dilution assay and bioautography method were used to evaluate the antibacterial activity of the extracts against Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis and Staphylococcus aureus and the antifungal activity against Candida albicans and Cryptococcus neoformans. Results: Methanol was the best extractant, yielding a larger amount of plant material whereas hexane yielded the least amount. In phytochemical analyses, more compounds were observed in BEA, followed by EMW and CEF. Qualitative 2, 2- diphenylpacryl-1-hydrazyl (DPPH) assay displayed that all the extracts had antioxidant activity. Antioxidant compounds could not be separated using BEA solvent system while with CEF and EMW enabled antioxidant compounds separation. The minimum inhibitory concentrations (MIC) values against test bacteria ranged between 0.16 and 2.50 mg/mL whereas against fungi, MIC ranged from 0.16 to 0.63 mg/mL. Bioautography results demonstrated that more than one compound was responsible for antimicrobial activity in the microdilution assay as the compounds were located at different Rf values. Conclusions: The results indicate that leaf extracts of Olea africana contain compounds with antioxidant, antibacterial and antifungal activities. Therefore, further studies are required to isolate the active compounds and perform other tests such as cytotoxicity. Olea africana may be a potential source of antimicrobial compounds. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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