Test-retest reproducibility of [C]PBR28 binding to TSPO in healthy control subjects.
Purpose: The PET radioligand [C]PBR28 binds to the translocator protein (TSPO), a marker of brain immune activation. We examined the reproducibility of [C]PBR28 binding in healthy subjects with quantification on a regional and voxel-by-voxel basis. In addition, we performed a preliminary analysis of...
| Publicado en: | European Journal of Nuclear Medicine & Molecular Imaging Vol. 43; no. 1; pp. 173 - 184 |
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| Autores principales: | , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Jan2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=111657747&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 111657747 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 16197070 NPC jtl: European Journal of Nuclear Medicine & Molecular Imaging issn: 16197070 maglogo: N pubinfo: dt: Jan2016 vid: 43 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 111657747 10.1007/s00259-015-3149-8 111657747 ppf: 173 ppct: 11 formats: fmt: @attributes: type: P tig: atl: Test-retest reproducibility of [C]PBR28 binding to TSPO in healthy control subjects. aug: au: Collste, K. Forsberg, A. Varrone, A. Amini, N. Aeinehband, S. Yakushev, I. Halldin, C. Farde, L. Cervenka, S. affil: Department of Clinical Neuroscience, Centre for Psychiatry Research, Karolinska Institutet, Stockholm Sweden sug: ab: Purpose: The PET radioligand [C]PBR28 binds to the translocator protein (TSPO), a marker of brain immune activation. We examined the reproducibility of [C]PBR28 binding in healthy subjects with quantification on a regional and voxel-by-voxel basis. In addition, we performed a preliminary analysis of diurnal changes in TSPO availability. Methods: Twelve subjects were examined using a high-resolution research tomograph and [C]PBR28, six in the morning and afternoon of the same day, and six in the morning on two separate days. Regional volumes of distribution ( V) were derived using a region-of-interest based two-tissue compartmental analysis (2TCM), as well as a parametric approach. Metabolite-corrected arterial plasma was used as input function. Results: For the whole sample, the mean absolute variability in V in the grey matter (GM) was 18.3 ± 12.7 %. Intraclass correlation coefficients in GM regions ranged from 0.90 to 0.94. Reducing the time of analysis from 91 to 63 min yielded a variability of 16.9 ± 14.9 %. There was a strong correlation between the parametric and 2TCM-derived GM values ( r = 0.99). A significant increase in GM V was observed between the morning and afternoon examinations when using secondary methods of quantification ( p = 0.028). In the subjects examined at the same time of the day, the absolute variability was 15.9 ± 12.2 % for the 91-min 2TCM data. Conclusion: V of [C]PBR28 binding showed medium reproducibility and high reliability in GM regions. Our findings support the use of parametric approaches for determining [C]PBR28 V values, and indicate that the acquisition time could be shortened. Diurnal changes in TSPO binding in the brain may be a potential confounder in clinical studies and should be investigated further. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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