Regulation of SCN3B/scn3b by Interleukin 2 (IL-2): IL-2 modulates SCN3B/scn3b transcript expression and increases sodium current in myocardial cells.
Background: In the initiation and maintenance of arrhythmia, inflammatory processes play an important role. IL-2 is a pro-inflammatory factor which is associated with the morbidity of arrhythmias, however, how IL-2 affects the cardiac electrophysiology is still unknown.Methods: In the present study,...
| Publicado en: | BMC Cardiovascular Disorders Vol. 16; pp. 1 - 8 |
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| Autores principales: | , , , , , , , , , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
BioMed Central
1/4/2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=112086398&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 112086398 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14712261 1CHA jtl: BMC Cardiovascular Disorders issn: 14712261 maglogo: N pubinfo: dt: 1/4/2016 vid: 16 pid: 24147 pub: BioMed Central artinfo: ui: 112086398 112086398 NLM26728597 112086398 10.1186/s12872-015-0179-x NLM26728597 PMC4700781 112086398 ppf: 1 ppct: 7 formats: tig: atl: Regulation of SCN3B/scn3b by Interleukin 2 (IL-2): IL-2 modulates SCN3B/scn3b transcript expression and increases sodium current in myocardial cells. aug: au: Yuanyuan Zhao Qiaobing Sun Zhipeng Zeng Qianqian Li Shiyuan Zhou Mengchen Zhou Yumei Xue Xiang Cheng Yunlong Xia Qing Wang Xin Tu Zhao, Yuanyuan Sun, Qiaobing Zeng, Zhipeng Li, Qianqian Zhou, Shiyuan Zhou, Mengchen Xue, Yumei Cheng, Xiang Xia, Yunlong affil: Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research, Cardio-X Institute, Huazhong University of Science and Technology, Wuhan 430074, China sug: subj: RNA Pharmacodynamics Interleukin 2 Drug Effects Genes Calcium Drug Effects Cells Metabolism Sodium Drug Effects Sodium Metabolism Genes Drug Effects Epithelial Cells Drug Effects Potassium Metabolism Cells Reverse Transcriptase Polymerase Chain Reaction Potassium Drug Effects Calcium Metabolism Sodium Pharmacodynamics RNA Cells Drug Effects Epithelial Cells Potassium Pharmacodynamics Membrane Potentials RNA Drug Effects Cytological Techniques Epithelial Cells Metabolism Interleukin 2 Metabolism Calcium Pharmacodynamics Interleukin 2 Pharmacodynamics Membrane Potentials Drug Effects Sodium Potassium RNA Metabolism Calcium Interleukin 2 ab: Background: In the initiation and maintenance of arrhythmia, inflammatory processes play an important role. IL-2 is a pro-inflammatory factor which is associated with the morbidity of arrhythmias, however, how IL-2 affects the cardiac electrophysiology is still unknown.Methods: In the present study, we observed the effect of IL-2 by qRT-PCR on the transcription of ion channel genes including SCN2A, SCN3A, SCN4A, SCN5A, SCN9A, SCN10A, SCN1B, SCN2B, SCN3B, KCNN1, KCNJ5, KCNE1, KCNE2, KCNE3, KCND3, KCNQ1, KCNA5, KCNH2 and CACNA1C. Western blot assays and electrophysiological studies were performed to demonstrate the effect of IL-2 on the translation of SCN3B/scn3b and sodium currents.Results: The results showed that transcriptional level of SCN3B was up-regulated significantly in Hela cells (3.28-fold, p = 0.022 compared with the control group). Consistent results were verified in HL-1 cells (3.73-fold, p = 0.012 compared with the control group). The result of electrophysiological studies showed that sodium current density increased significantly in cells which treated by IL-2 and the effect of IL-2 on sodium currents was independent of SCN3B (1.4 folds, p = 0.023). Western blot analysis showed IL-2 lead to the significantly increasing of p53 and scn3b (2.1 folds, p = 0.021 for p53; 3.1 folds, p = 0.023 for scn3b) in HL-1 cells. Consistent results were showed in HEK293 cells using qRT-PCR analysis (1.43 folds for P53, p = 0.022; 1.57 folds for SCN3B, p = 0.05).Conclusions: The present study suggested that IL-2, may play role in the arrhythmia by regulating the expression of SCN3B and sodium current density. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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