Al[18F]NOTA-T140 Peptide for Noninvasive Visualization of CXCR4 Expression.
Purpose: Chemokine receptor CXCR4 plays an important role in tumor aggressiveness, invasiveness, and metastasis formation. Quantification of CXCR4 expression by tumors may have an impact on prediction and evaluation of tumor response to therapies. In this study, we developed a robust and straightfor...
| Publicado en: | Molecular Imaging & Biology Vol. 18; no. 1; pp. 135 - 143 |
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| Autores principales: | , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Feb2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=112404315&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 112404315 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Feb2016 vid: 18 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 112404315 112404315 NLM26126597 112404315 10.1007/s11307-015-0872-2 NLM26126597 112404315 ppf: 135 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Al[18F]NOTA-T140 Peptide for Noninvasive Visualization of CXCR4 Expression. aug: au: Yan, Xuefeng Niu, Gang Wang, Zhe Yang, Xiangyu Kiesewetter, Dale Jacobson, Orit Shen, Baozhong Chen, Xiaoyuan Kiesewetter, Dale O affil: Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda USA sug: subj: Fluorine Radioisotopes Metabolism Receptors, Cell Surface Metabolism Aluminum Metabolism Oligopeptides Metabolism Heterocyclic Compounds Metabolism Hamsters Animal Studies Oligopeptides Cytokines Metabolism Rodents Mice Neoplasm Metastasis Heterocyclic Compounds Tomography, Emission-Computed Binding Sites Funding Source ab: Purpose: Chemokine receptor CXCR4 plays an important role in tumor aggressiveness, invasiveness, and metastasis formation. Quantification of CXCR4 expression by tumors may have an impact on prediction and evaluation of tumor response to therapies. In this study, we developed a robust and straightforward F-18 labeling route of T140, a CXCR4 peptide-based antagonist.Procedures: T140 derivative was conjugated to 1,4,7-triazacyclononane-triacetic acid (NOTA) and labeled with Al[(18)F]. Al[(18)F]NOTA-T140 was evaluated in vitro in cell-based assay and stability in mouse serum and in vivo using CXCR4 positive and negative tumor xenograft models.Results: Labeling of Al[(18)F]NOTA-T140 was completed within 30 min with a radiochemical yield of 58 ± 5.3 % at the end of synthesis, based on fluoride-18 activity. Al[(18)F]NOTA-T140 accumulated in CHO-CXCR4 positive but not negative tumors. Al[(18)F]NOTA-T140 uptake in the tumors correlated with CXCR4 protein expression. Moreover, Al[(18)F]NOTA-T140 had high accumulation in CXCR4-positive metastatic tumors.Conclusions: The simplicity of Al[(18)F]NOTA-T140 labeling along with its properties to specifically image CXCR4 expression by tumors warrant further clinical application for the diagnosis of CXCR4 clinically. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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