Preclinical Evaluation of 4-[18F]Fluoroglutamine PET to Assess ASCT2 Expression in Lung Cancer.
Purpose: Alanine-serine-cysteine transporter 2 (ASCT2) expression has been demonstrated as a promising lung cancer biomarker. (2S,4R)-4-[(18)F]Fluoroglutamine (4-[(18)F]fluoro-Gln) positron emission tomography (PET) was evaluated in preclinical models of non-small cell lung cancer as a quantitative,...
| Publicado en: | Molecular Imaging & Biology Vol. 18; no. 1; pp. 18 - 24 |
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| Autores principales: | , , , , , , , , , , , , , , , , , |
| Formato: | diagnostic images pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Feb2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=112404323&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 112404323 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Feb2016 vid: 18 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 112404323 112404323 NLM25971659 112404323 10.1007/s11307-015-0862-4 NLM25971659 PMC4644503 112404323 ppf: 18 ppct: 6 formats: fmt: @attributes: type: P tig: atl: Preclinical Evaluation of 4-[18F]Fluoroglutamine PET to Assess ASCT2 Expression in Lung Cancer. aug: au: Hassanein, Mohamed Hight, Matthew Buck, Jason Tantawy, Mohammed Nickels, Michael Hoeksema, Megan Harris, Bradford Boyd, Kelli Massion, Pierre Manning, H. Hight, Matthew R Buck, Jason R Tantawy, Mohammed N Nickels, Michael L Hoeksema, Megan D Harris, Bradford K Massion, Pierre P Manning, H Charles affil: Division of Allergy, Pulmonary and Critical Care Medicine, Vanderbilt University School of Medicine, Nashville 37232 USA sug: subj: Glutamine Lung Neoplasms Metabolism Tomography, Emission-Computed Methods Lung Neoplasms Radiography Carrier Proteins Metabolism Colonic Neoplasms Radiography Carcinoma, Non-Small-Cell Lung Metabolism Cell Line, Tumor Animal Studies Glutamine Metabolism Carcinoma, Non-Small-Cell Lung Radiography Colonic Neoplasms Metabolism Epidermal Growth Factors Mutation Mice Funding Source ab: Purpose: Alanine-serine-cysteine transporter 2 (ASCT2) expression has been demonstrated as a promising lung cancer biomarker. (2S,4R)-4-[(18)F]Fluoroglutamine (4-[(18)F]fluoro-Gln) positron emission tomography (PET) was evaluated in preclinical models of non-small cell lung cancer as a quantitative, non-invasive measure of ASCT2 expression.Procedures: In vivo microPET studies of 4-[(18)F]fluoro-Gln uptake were undertaken in human cell line xenograft tumor-bearing mice of varying ASCT2 levels, followed by a genetically engineered mouse model of epidermal growth factor receptor (EGFR)-mutant lung cancer. The relationship between a tracer accumulation and ASCT2 levels in tumors was evaluated by IHC and immunoblotting.Result: 4-[(18)F]Fluoro-Gln uptake, but not 2-deoxy-2-[(18)F]fluoro-D-glucose, correlated with relative ASCT2 levels in xenograft tumors. In genetically engineered mice, 4-[(18)F]fluoro-Gln accumulation was significantly elevated in lung tumors, relative to normal lung and cardiac tissues.Conclusions: 4-[(18)F]Fluoro-Gln PET appears to provide a non-invasive measure of ASCT2 expression. Given the potential of ASCT2 as a lung cancer biomarker, this and other tracers reflecting ASCT2 levels could emerge as precision imaging diagnostics in this setting. pubtype: Academic Journal doctype: diagnostic images pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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