Low-Level Vagus Nerve Stimulation Attenuates Myocardial Ischemic Reperfusion Injury by Antioxidative Stress and Antiapoptosis Reactions in Canines.
Vagus Nerve Stimulation and Myocardial Ischemia-Reperfusion Injury Background Low-level vagus nerve stimulation (LL-VNS) has been demonstrated to protect myocardium against acute ischemia/reperfusion (I/R) injury. However, the underlying mechanism of this protective effect remains unknown. Objective...
| Publicado en: | Journal of Cardiovascular Electrophysiology Vol. 27; no. 2; pp. 224 - 232 |
|---|---|
| Autores principales: | , , , , , , , , |
| Formato: | pictorial research tables/charts tracings Journal Article |
| Publicado: |
Wiley-Blackwell
Feb2016
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=112536091&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 112536091 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10453873 GSB jtl: Journal of Cardiovascular Electrophysiology issn: 10453873 maglogo: Y pubinfo: dt: Feb2016 vid: 27 iid: 2 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 112536091 112536091 112536091 10.1111/jce.12850 112536091 ppf: 224 ppct: 8 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Low-Level Vagus Nerve Stimulation Attenuates Myocardial Ischemic Reperfusion Injury by Antioxidative Stress and Antiapoptosis Reactions in Canines. aug: au: CHEN, MINGXIAN ZHOU, XIAOYA YU, LILEI LIU, QIMING SHENG, XIA WANG, ZHUO WANG, SONGYUN JIANG, HONG ZHOU, SHENGHUA affil: Department of Cardiology, The Second Xiangya Hospital of Central South University, Changsha Hunan, China sug: subj: Vagal Stimulation Utilization Reperfusion Injury Prevention and Control Myocardial Ischemia Prevention and Control Animal Studies Dogs Random Assignment Dyes Diagnostic Use Funding Source Oxidative Stress Physiology Heart Rate Variability Spectral Analysis Descriptive Statistics Data Analysis Software P-Value Confidence Intervals Odds Ratio Algorithms Severity of Injury Evaluation Myocardial Infarction Neutrophils Immunology Study Design T-Tests Fisher's Exact Test One-Way Analysis of Variance Staining and Labeling Apoptosis Physiology Gene Expression Blotting, Western Utilization Oncogenes Physiology Proteins Physiology Histocytochemistry Methods ab: Vagus Nerve Stimulation and Myocardial Ischemia-Reperfusion Injury Background Low-level vagus nerve stimulation (LL-VNS) has been demonstrated to protect myocardium against acute ischemia/reperfusion (I/R) injury. However, the underlying mechanism of this protective effect remains unknown. Objective This study aimed to test the hypothesis that LL-VNS exerts cardioprotective effect on acute I/R injury in canines via antioxidative stress and antiapoptosis reactions. Method Thirty anesthetized mongrel dogs were randomly divided into three groups: I/R group (N = 12, the left anterior descending coronary artery was occluded for 1 hour following by 1 hour reperfusion), LL-VNS group (N = 9, I/R plus LL-VNS), and sham group (N = 9, sham surgery without LL-VNS). The voltage threshold was set at 80% of the voltage required to slow the sinus rate. Infarct size was assessed with Evans Blue and triphenyltetrazolium chloride. Activity assays, TUNEL staining, and western blotting were performed to determine markers of oxidative stress and apoptosis. Results LL-VNS significantly decreased the incidence of ventricular arrhythmias, increased vagal tone, as confirmed by heart rate viability, and reduced infarct size compared with the I/R group. This improvement was associated with a reduction in myocardial neutrophil infiltration, the inhibition of oxidative stress, and the suppression in cardiomyocyte apoptosis. In contrast, the lack of LL-VNS in the I/R group induced the opposite effect compared with the sham group. Conclusion LL-VNS exerts protective effects on myocardial I/R injury. Its potential mechanisms involve the suppression of oxidative stress and cellular apoptosis. pubtype: Academic Journal doctype: pictorial research tables/charts tracings Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|