Skeletal muscles respond differently when piglets are offered a diet 30 % deficient in total sulfur amino acid for 10 days.
Purpose: Although amino acids (AA) are required for growth, little is known about the effect of a deficient AA supply on the composition and the contractile and metabolic properties of skeletal muscles. Methods: Protein metabolism, oxidative catabolism, glutathione system, and fiber-type composition...
| Publicado en: | European Journal of Nutrition Vol. 55; no. 1; pp. 117 - 127 |
|---|---|
| Autores principales: | , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Springer Nature
Feb2016
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=112692640&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 112692640 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Feb2016 vid: 55 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 112692640 112692640 112692640 10.1007/s00394-014-0830-9 112692640 ppf: 117 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Skeletal muscles respond differently when piglets are offered a diet 30 % deficient in total sulfur amino acid for 10 days. aug: au: Conde-Aguilera, José Lefaucheur, Louis Tesseraud, Sophie Mercier, Yves Floc'h, Nathalie Milgen, Jaap affil: INRA, UR83 Recherches Avicoles, 37380 Nouzilly France sug: subj: Amino Acids, Sulfur Deficiency Muscle, Skeletal Physiology Muscle, Skeletal Anatomy and Histology France Animal Studies In Vivo Studies Swine Funding Source Muscle Proteins Analysis Oxidative Stress Glutathione Analysis Muscle Fibers Physiology Amino Acids, Branched-Chain Analysis Enzymes Analysis Muscle, Skeletal Analysis Lactate Dehydrogenase Analysis Methionine Analysis Histological Techniques Peptide Hydrolases Analysis Two-Way Analysis of Variance Data Analysis Software One-Way Analysis of Variance Post Hoc Analysis Descriptive Statistics P-Value ab: Purpose: Although amino acids (AA) are required for growth, little is known about the effect of a deficient AA supply on the composition and the contractile and metabolic properties of skeletal muscles. Methods: Protein metabolism, oxidative catabolism, glutathione system, and fiber-type composition of the longissimus (LM), rhomboideus (RM), and semitendinous (SM) muscles were compared between 42-day-old piglets pair-fed for 10 days either with a diet with a 28 % deficient supply of total sulfur AA (TSAA−) or with a diet with a sufficient supply of total sulfur AA (TSAA+). Results: The relative weight, protein mass, and protein synthesis of LM were 10-32 % lower in TSAA− pigs compared with TSAA+ pigs, while RM and SM were not affected by the TSAA supply. The TSAA supply affected the AA composition of muscles. Concentrations of Met and branched-chain AA were, respectively, 7 and 3 % lower in TSAA− pigs compared with TSAA+ pigs. The His concentration was 30 % higher in LM and SM in TSAA− pigs compared with TSAA+ pigs and unaffected in RM. The activity of citrate synthase was 14 % higher in all three muscles of TSAA− pigs. In these pigs, the β-hydroxy-acyl-CoA dehydrogenase activity was 20 % higher in RM compared with TSAA+ pigs while that of lactate dehydrogenase was 21 % lower in LM. Total and reduced glutathione concentrations were more than 70 % greater in RM than in LM or SM, and these concentrations were approximately 10 % lower in TSAA− pigs than in TSAA+ pigs. Conclusions: Results of this study indicate that a TSAA deficiency affects muscle properties in a muscle-dependent manner increasing the oxidative capacity of RM and reducing growth and glycolytic metabolism of LM. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|