Epidermal growth factor receptor mutations in nonsmall cell lung carcinoma patients in Kuwait.
Context: Nonsmall cell lung carcinoma (NSCLC) is the most frequently diagnosed form of lung cancer in Kuwait. NSCLC samples from Kuwait have never been screened for epidermal growth factor receptor (EGFR) gene aberration, which is known to affect treatment options. Aims: This study investigated the...
| Publicado en: | Journal of Cytology Vol. 33; no. 1; pp. 1 - 7 |
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| Autores principales: | , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wolters Kluwer India Pvt Ltd
Jan-Mar2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=112809032&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 112809032 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09709371 58DU jtl: Journal of Cytology issn: 09709371 maglogo: N pubinfo: dt: Jan-Mar2016 vid: 33 iid: 1 pid: 16919 pub: Wolters Kluwer India Pvt Ltd artinfo: ui: 112809032 112809032 112809032 10.4103/0970-9371.175476 112809032 ppf: 1 ppct: 6 formats: tig: atl: Epidermal growth factor receptor mutations in nonsmall cell lung carcinoma patients in Kuwait. aug: au: AL-TEMAIMI, RABEAH KAPILA, KUSUM AL-MULLA, FAHD R. FRANCIS, ISSAM M. AL-WAHEEB, SALAH AL-AYADHY, BUSHRA affil: Department of Pathology, Human Genetics Unit, Kuwait University, Safat, Kuwait sug: subj: Epidermal Growth Factors Mutation Chemoreceptor Cells Carcinoma, Non-Small-Cell Lung Diagnosis Carcinoma, Non-Small-Cell Lung Familial and Genetic Human Kuwait Immunohistochemistry Biopsy, Needle Adult Middle Age Aged Aged, 80 and Over Male Female Funding Source Adult: 19-44 years Middle Aged: 45-64 years Aged: 65+ years Aged, 80 & over Male Female ab: Context: Nonsmall cell lung carcinoma (NSCLC) is the most frequently diagnosed form of lung cancer in Kuwait. NSCLC samples from Kuwait have never been screened for epidermal growth factor receptor (EGFR) gene aberration, which is known to affect treatment options. Aims: This study investigated the feasibility of using fi ne-needle aspiration (FNA) material for mutational screening, and whether common EGFR mutations are present in NSCLC samples from Kuwait. Settings and Design: Eighteen NSCLC samples from fi ve Kuwaitis and 13 non-Kuwaitis were included in this study. Materials and Methods: DNA was extracted from FNA cell blocks and screened for EGFR gene mutations using peptide nucleic acid (PNA)-clamp assay, and EGFR gene amplifi cation using fl uorescent in situ hybridization (EGFR-FISH). EGFR protein expression was assessed using immunohistochemistry. Results: Five EGFR mutations were detected in fi ve non-Kuwaiti NSCLC patients (27.8%). EGFR gene amplifi cation was evident in 10 samples (55.5%) by direct amplifi cation or under the infl uence of chromosomal polysomy. Four samples had EGFR mutations and EGFR gene amplifi cation, out of which only one sample had coexisting EGFR overexpression. Conclusions: Given the evidence of EGFR gene alterations occurring in NSCLC patients in Kuwait, there is a need to incorporate EGFR gene mutational screen for NSCLC patients to implement its consequent use in patient treatment. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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