MAPK-mediated enhanced expression of vacuolar H-ATPase confers the improved adaption to NaCl stress in a halotolerate peppermint ( Mentha piperita L.).
Vacuolar H-ATPase (V-H-ATPase) has been proved to be of importance in maintenance of ion homeostasis inside plant cells under NaCl stress. In this study, the expression levels and salt-tolerate function of V-H-ATPase genes were investigated in the roots and leaves of a halotolerate peppermint ( Ment...
| Publicado en: | Protoplasma Vol. 253; no. 2; pp. 553 - 570 |
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| Autores principales: | , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Mar2016
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=113576236&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 113576236 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0033183X 2CF jtl: Protoplasma issn: 0033183X maglogo: N pubinfo: dt: Mar2016 vid: 253 iid: 2 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 113576236 10.1007/s00709-015-0834-1 113576236 ppf: 553 ppct: 17 formats: fmt: @attributes: type: P tig: atl: MAPK-mediated enhanced expression of vacuolar H-ATPase confers the improved adaption to NaCl stress in a halotolerate peppermint ( Mentha piperita L.). aug: au: Li, Zhe Zhen, Zhen Guo, Kai Harvey, Paul Li, Jishun Yang, Hetong affil: Biotechnology Center, Shandong Academy of Sciences, Jinan 250014 China sug: ab: Vacuolar H-ATPase (V-H-ATPase) has been proved to be of importance in maintenance of ion homeostasis inside plant cells under NaCl stress. In this study, the expression levels and salt-tolerate function of V-H-ATPase genes were investigated in the roots and leaves of a halotolerate peppermint ( Mentha × piperita L.) Keyuan-1 treated with different concentrations of NaCl. Results showed that the expressions of V-H-ATPase in the transcriptional, protein and activity levels were significantly enhanced in the halotolerate peppermint Keyuan-1 compared to the wild-type (WT) peppermint under 50, 100, and 150 mM NaCl treatment. Moreover, inhibition experiments exhibited that V-H-ATPase activity played vital roles in the salt tolerance of peppermint Keyuan-1 to 150 mM NaCl stress through increasing the vacuolar H pumping activity and Na compartmentalization capacity. Furthermore, results of Western blots showed that the activity of a mitogen-activated protein kinase (MAPK) was significantly increased under different concentrations of NaCl in the halotolerate peppermint Keyuan-1, which was much higher than that of WT peppermint. Further experiments with inhibitors suggested that this MAPK protein was responsible for the enhanced expression of V-H-ATPase in the halotolerate peppermint Keyuan-1. In response to NaCl stress, increase of cytoplasmic calcium concentration ([Ca]) occurred upstream of MAPK activation in the halotolerate peppermint Keyuan-1. In all, these findings demonstrated that increased V-H-ATPase activity was positively correlated with the enhanced salt tolerance in the halotolerate peppermint Keyuan-1, providing the theoretic basis for the further development and utilization of peppermint in saline areas. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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